| 盐爪爪核酸提取方法研究 |
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| 引用本文： | 贾晋,蔡禄,施聪. 盐爪爪核酸提取方法研究[J]. 中国农学通报, 2010, 26(4): 49-52 |
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| 作者姓名： | 贾晋 蔡禄 施聪 |
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| 作者单位： | 内蒙古科技大学数理与生物工程学院,内蒙古包头,014010 |
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| 基金项目： | 内蒙古教育厅基金"植物抗(耐)盐碱基因的克隆" |
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| 摘 要： | 土壤盐渍化是一个世界性的生态问题，与传统的水利工程改良措施相比，挖掘盐生植物的生物潜力对实现盐渍土的生物改良和有效利用具有重要作用。本实验所用材料为藜科植物盐爪爪，提取高质量的核酸是对盐爪爪进行分子研究的前提和基础，也是进行植物耐盐基因工程研究的基础。因此，本实验研究了盐爪爪核酸的提取方法，所得结果为盐爪爪耐盐机理的进一步研究奠定基础，也为其它植物的核酸提取提供借鉴。
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| 关 键 词： | 3%多抗霉素水剂 3%多抗霉素水剂 苹果落叶半点病 药效试验 |
| 收稿时间： | 2009-09-14 |
| 修稿时间： | 2009-11-01 |
Study on extraction method of nucleic acid in Kalidium foliatum (Pall.) Moq. |
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| Jia Jin,Cai Lu,Shi Cong. Study on extraction method of nucleic acid in Kalidium foliatum (Pall.) Moq.[J]. Chinese Agricultural Science Bulletin, 2010, 26(4): 49-52 |
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| Authors: | Jia Jin Cai Lu Shi Cong |
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| Affiliation: | Jia Jin,Cai Lu,Shi Cong(College of Mathematics,Physics , Chemical Engineering,Inner Mongolia University of Science&technology,Baotou Inner Mongolia 014010) |
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| Abstract: | In order to extracting high-quality nucleic acid in Kalidium foliatum(Pall.)Moq.,and to laying a foundation for its gene engineering research,biology improvement of saline soil and resolving soil salinization-an ecological problem in a world,The CTAB,SDS and improved urine method were adopted to extracting DNA of Kalidium foliatum(Pall.)Moq.,and added a step which employ alcohol and LiCl to precipitate particularly RNA,RT-PCR technology was adopted to examine quality of the RNA from SDS-LiCl method;Research... |
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| Keywords: | RT-PCR |
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