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| 牛奶尿素氮测定方法研究 | |
| 引用本文： | 黄文明,李胜利,曹志军,王之盛.牛奶尿素氮测定方法研究[J].中国畜牧杂志,2009,45(9). |
| 作者姓名： | 黄文明 李胜利 曹志军 王之盛 |
| 作者单位： | 1. 中国农业大学动物科技学院,北京,100193;四川农业大学动物营养研究所,四川,雅安,625014 2. 中国农业大学动物科技学院,北京,100193 3. 四川农业大学动物营养研究所,四川,雅安,625014 |
| 基金项目： | 中国农业大学-南京农业大学青年教师开放基金，公益性行业(农业)科研专项经费资助项目 |
| 摘 要： | 本试验结合二乙酰-肟试剂盒法探讨牛奶尿素氮的测定方法。结果表明:对牛奶样品进行1500×g离心10min脱脂,再3500×g离心20min脱蛋白的前处理与不脱脂,3500×g离心20min脱蛋白的前处理相比,牛奶尿素氮测定值差异极显著(P<0.01),与不脱脂,5000×g离心20min脱蛋白的前处理相比,牛奶尿素氮测定值差异不显著(P>0.05)。牛奶样品不经过脱脂过程,5000×g离心10min和15min与离心20min牛奶尿素氮测定值差异不显著(P>0.05),但离心10min的牛奶尿素氮测定值不稳定。研究结果表明,牛奶样品可以不脱脂,经5000×g离心15min脱蛋白后测定牛奶尿素氮含量。 |
| 关 键 词： | 尿素氮 二乙酰-肟法 测定方法 牛奶 |
A Methodological Study on the Determination of Milk Urea Nitrogen |
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| HUANG Wen-ming,LI Sheng-li,CAO Zhi-jun,WANG Zhi-Sheng.A Methodological Study on the Determination of Milk Urea Nitrogen[J].Chinese Journal of Animal Science,2009,45(9). | |
| Authors: | HUANG Wen-ming LI Sheng-li CAO Zhi-jun WANG Zhi-Sheng |
| Abstract: | The experiment was conducted to discuss the measurement of milk urea nitrogen ( MUN) with diacetyl-oxime kit. The result showed that centrifuging at 1,500×g for 10 mins to degrease and at 3,500×g for 20 mins to deprotein had a significant difference in not degreasing and centrifuging at 3,500×g for 20 mins to deprotein, and there was not significant difference in not degreasing and centrifuging at 5,000 ×g for 20 mins to deprotein. There was not significant difference between centrifuging at 5,000×g for 10 mins,15 mins and 20 mins, but the results of centrifuging for 10 mins were unstable. These results indicated that centrifuging at 5,000×g for 15 mins and not degreasing could measure content of MUN. |
| Keywords: | MUN diacetyl-oxime methodology milk |
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