| 沙冬青cDNA-AFLP体系的建立及引物筛选 |
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| 引用本文： | 王丹丹,尹伟伦,夏新莉.沙冬青cDNA-AFLP体系的建立及引物筛选[J].安徽农业科学,2009,37(24):11446-11449. |
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| 作者姓名： | 王丹丹 尹伟伦 夏新莉 |
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| 作者单位： | 北京林业大学生物科学与技术学院,北京,100083;北京林业大学生物科学与技术学院,北京,100083;北京林业大学生物科学与技术学院,北京,100083 |
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| 摘 要： | 目的]建立适合于沙冬青cDNA—AFLP的反应体系。方法]以对照、3种逆境处理的沙冬青为材料，采用Trizol法和改良CTAB法提取总RNA。结果]改良的CTAB法可抽提到较高质量的总RNA，适于沙冬青总RNA的提取。采用EcoRI/MseI酶切体系，64对选择性扩增引物中有35对适合沙冬青cDNA-AFLP分析。结论]该研究为利用cDNA-AFLP标记对沙冬青抗逆基因进行深入研究奠定基础。
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| 关 键 词： | 沙冬青 RNA提取 cDNA-AFLP |
Establishment of a cDNA-AFLP System in Ammopiptanthus mongolicus and Primer Screening |
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| WANG Dan-dan et al.Establishment of a cDNA-AFLP System in Ammopiptanthus mongolicus and Primer Screening[J].Journal of Anhui Agricultural Sciences,2009,37(24):11446-11449. |
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| Authors: | WANG Dan-dan |
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| Institution: | WANG Dan-dan et al (College of Biological Sciences , Biotechnology,Beijing Forestry University,Beijing 100083) |
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| Abstract: | Objective] The research aimed to establish the cDNA-AFLP system in Ammopiptanthus mongolicus.Method] RNA was extracted from the control and three stress-treatment materials.Trizol and CTAB method to extract RNA were compared.Result] Improved CTAB method could obtain a higher quality of the total RNA in Ammopiptanthus mongolicus.Using EcoR Ⅰ /Mse Ⅰ digestion system,35 selective amplification primer pairs were identified from 64 selective amplification primer pairs,which were suitable for cDNA-AFLP analysis.Conclusion] The research could provide important evidence for further studying the stress-response genes in Ammopiptanthus mongolicus. |
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| Keywords: | cDNA-AFLP |
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