| 产朊假丝酵母原生质体制备的研究 |
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| 引用本文： | 王立群,聂晶.产朊假丝酵母原生质体制备的研究[J].东北农业大学学报,1998,29(4):384-387. |
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| 作者姓名： | 王立群 聂晶 |
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| 作者单位： | [1]东北农业大学生物工程系 [2]哈尔滨市职工医学院 |
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| 摘 要： | 利用产朊假丝酵母（Ｃａｎｄｉｄａｕｔｉｌｉｓ）２．２８１株研究了原生质体的制备。结果表明，该菌经复合剂０．１４ｍｏｌ·Ｌ－１β－ＭＥＴ、０．０４ｍｏｌ·Ｌ－１ＥＤＴＡ予处理；蜗牛酶浓度为７０ｍｇ·（１０ｍＬ）－１；并于３０℃酶解１２０ｍｉｎ；是制备原生质体的理想条件
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| 关 键 词： | 原生质体，蜗牛酶，产朊假丝酵母 |
STUDIES ON MAKING CANDIDA UTILIS PROTPLAST |
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| Wang Liqun.STUDIES ON MAKING CANDIDA UTILIS PROTPLAST[J].Journal of Northeast Agricultural University,1998,29(4):384-387. |
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| Authors: | Wang Liqun |
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| Institution: | Wang Liqun (Northeast Agricultural University Harbin 150030 PRC) Nie Jing (Harbin Workers Medical Institue 150080 PRC) |
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| Abstract: | The results of studyon making Candida utilis 2.281 strain protoplast showed that the strain treated with 0.14 mol β-MET, 0.04 mol EDTA can be changed into protoplast well when the anounts of snailase is 70 mg·(10 ml) -1 , tenperature at 30℃, 120 min. |
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| Keywords: | protoplast snailase Gandida utilis |
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