江西胜红蓟黄脉病病原的分子鉴定

为明确引起江西赣州地区胜红蓟上黄脉症状的病原,利用PCR技术,从3个样品上均扩增到双生病毒的约500 bp特异片段,并选择病毒分离物JX01进行全序列测定。结果表明,JX01 DNA-A全长2 754 nts,符合双生病毒DNA-A的结构特征,与中国番木瓜曲叶病毒(Papaya leaf curl China virus,Pa LCCNV)分离物Pa LCCNVFz10同源性最高,达99.7%。利用特异性引物对JX01样品进行卫星分子的扩增,得到大约1 300 bp的片段(JX-Gz01β)。序列分析表明,JX-Gz01β全长1 314 nts,与伴随中国胜红蓟黄脉病毒的卫星分子(Ageratum yellow vein China betasatellite,AYVCNB)Hn9分离物的同源性最高,为94.3%。系统关系树分析表明,JX01与Pa LCCNV各分离物聚类为一个大分支,而与其他病毒亲缘关系较远。这是江西首次报道Pa LCCNV侵染胜红蓟。

Molecular Identification of the Pathogen Inducing Ageratum Yellow Vein Disease in Jiangxi

To determine the virus species infecting Ageratum conyzoides in Ganzhou, Jiangxi Province, weed samples showing typical geminivirus?like symptoms were tested by PCR technique.A fragment of 500 bp was consistently amplified from total DNA extracts of 3 samples with a pair of specific primer.Comparison of se?quences of the 500 bp fragment revealed that these samples were infected by a same virus and thus an isolate denoted JX01 was selected for further sequence analysis.The complete sequence of JX01 comprised 2 754 nu?cleotides with typical genomic organization of geminiviral DNA?A.JX01 was most closely related to Papaya leaf curl China virus isolate Fz10 ( PaLCCNV?Fz10) ,with 99.7% nucleotide sequence identity.By using the specific primers for the full length of betasatellite,a fragment of about 1 300 bp was amplified.The complete sequence of betasatellite (JX?Gz01β) was determined to be 1 314 nucleotides.Sequence analysis showed that JX?Gz01βwas most closely related to Ageratum yellow vein China betasatellite isolate Hn9 (AYVCNB?Hn9),with 94.3% nu?cleotide sequence identity.Phylogenetic analysis showed that JX01 clustered together with isolates of PaLCCNV. This is the first report of PaLCCNV infecting Ageratum conyzoides in Jiangxi Province.