山药种质包埋玻璃化超低温保存再生植株的稳定性分析

为了验证前期建立的包埋玻璃化超低温保存技术是山药(Dioscorea opposita)种质资源离体保存的一种有效的方法,对包埋玻璃化超低温保存后的山药再生植株的形态、生理和同工酶酶谱进行了研究。结果表明:与常温保存的植株相比,供试的铁棍山药再生植株的平均株高、叶片数、芽数和茎节长,叶片中叶绿素、可溶性蛋白和可溶性糖含量以及超氧化物歧化酶(SOD)活力在绝对值上虽有差异,但均未达到显著水平,且能移栽成活,保持了形态和生理的稳定性;供试的铁棍、太谷、怀庆和嘉祥细长毛4种山药再生植株的SOD和蛋白水解酶的同工酶条带数目及强度均未发生变化,保持了酶蛋白的稳定性。说明前期建立的包埋玻璃化法超低温保存技术是山药种质离体保存的一种有效方法。

Stability Analysis of Regenerated Plantlets from Dioscorea opposita Germplasm Cryopreserved by Encapsulation-vitrification

In order to verify that cryopreservation technology with encapsulation-vitrification established in an early stage is an effective method of preserving Dioscorea opposita germplasm.From the morphology,physiology and zymogram of isoenzyme,stability analyses of regenerated plantlets from Dioscorea opposita germplasm cryopreserved by encapsulation-vitrification were studied in this paper.The results showed that:compared to plantlets at room temperature,the absolute values of average height,average leaf number,average bud number,average node length,and chlorophyll,soluble protein and soluble sugar contents,superoxide dismutase(SOD)activity of regenerated plantlets from D.opp.cv.Tiegun had all not significantly difference,and the regenerated plantlets could survive after transplanting.In a word,they kept stability of morphology and physiology.Among 4 tested yam cultivars(that is Tiegun,Taigu,Huaiqing and Jiaxiangxichangmao),SOD and protease zymogram,including the band number and strength,there was no difference between regenerated plantlets without cryopreservation and with cryopreservation by encapsulation-vitrification.Cryopreservation by encapsulation-vitrification could keep up stability and be taken as an effective method of Dioscorea opposita germplasm in vitro preservation.