人参属植物鲨烯合酶编码基因及其氨基酸序列的生物信息学分析

利用生物信息学方法对GenBank中人参属9种植物鲨烯合酶的cDNA序列以及其编码的氨基酸序列的结构、理化性质、信号肽、疏水性、亲水性、亚细胞定位、跨膜结构域功能域和进化关系进行了初步预测和分析。结果表明：总体上人参属鲨烯合酶核苷酸序列相似性平均为96.245%，氨基酸相似性平均为95.5%。其二级结构预测结果显示9个鲨烯合酶氨基酸序列以α螺旋和无规则卷曲为主要组成部分。对9种人参属植物进行进化树分析发现可以分为2个亚族。对包括人参属9种植物在内的其他物种进行进化树分析可知，植物、动物、酵母分属不同的类群。通过分析人参属植物鲨烯合酶及其编码基因的生物信息学特征，可以为鲨烯合酶基因的克隆和遗传操作提供理论参考。

Bioinformatics Analysis of Squalene Synthase Coding Gene and Amino Acid Sequence in Ginsneg Genus

Nine Panax ginseng genus squalene synthase in Genebank was analyzed in this article.Squalene synthase cDNA sequence and its encoded amino acid sequence,physicochemical properties,the signal peptide,hydrophobic,hydrophilic,subcellular localization,transmembrane topological structure,function domain and evolutional relationship were predicted by using bioinformatics methods.The result showed the similarity of ginseng squalene synthase nucleotide was 96.245%,and the sequence of amino acid shared 95.5% similarity on average.Furthermore,the main composition of 9 amino acid sequences of squalene synthase were alpha helix and random coil via secondary structure predicted.Nine ginseng species could be divided into two subtribes by phylogenetic tree analysis.The phylogenetic tree which included nine ginseng plants and other species of plants,animals,yeast showed they belong to different groups.By analyzing the biological characteristics of ginseng squalene synthase,it could provide theoretical reference for the squalene synthase gene cloning and genetic manipulation.