| 高直链淀粉玉米amylose-extender基因功能标记的开发及应用 |
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| 引用本文： | 宁丽华,陈亭亭,刘怀华,刘旭,马侠,崔德周,姜川,张华,王莉雯,李德涛,陈化榜.高直链淀粉玉米amylose-extender基因功能标记的开发及应用[J].分子植物育种,2011,9(2):185-189. |
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| 作者姓名： | 宁丽华 陈亭亭 刘怀华 刘旭 马侠 崔德周 姜川 张华 王莉雯 李德涛 陈化榜 |
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| 作者单位： | 作物生物学国家重点实验室,山东省作物生物学重点实验室,山东农业大学农学院,泰安,271018 |
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| 基金项目： | 国家自然基金项目(31071424); 山东省良种工程重大课题鲁农良种字(2008)共同资助 |
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| 摘 要： | 直链淀粉的含量是玉米淀粉品质的重要指标,受隐性核基因amylose-extender(ae)控制.生产中直链淀粉含量的测定费时、费力,因此高直链淀粉玉米的改良需要寻找快速、简便、准确的基因检测方法.本实验对高直链淀粉玉米材料和普通玉米材料ae基因的cDNA进行了测序,结果显示高直链淀粉材料cDNA 序列第9外显子(84...
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| 关 键 词： | 玉米 高直链淀粉 功能标记 |
Development and Utilization of amylose-extender Functional Marker in High-amylose Maize |
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| Ning Lihua,Chen Tingting,Liu Huaihua,Liu Xu,Ma Xia,Cui Dezou,Jiang Chuan,Zhang Hua,WangLiwen,Li Detao,Chen Huabang.Development and Utilization of amylose-extender Functional Marker in High-amylose Maize[J].Molecular Plant Breeding,2011,9(2):185-189. |
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| Authors: | Ning Lihua Chen Tingting Liu Huaihua Liu Xu Ma Xia Cui Dezou Jiang Chuan Zhang Hua WangLiwen Li Detao Chen Huabang |
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| Institution: | ﹡ State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agriculture, Shandong Agricultural University, Taian, 271018 |
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| Abstract: | Amylose in maize, a recessive trait conferred by nuclear gene (ae), is one of the most important qualities of maize starch. Maize breeders have an interest in gaining access to a quick, precise and simple method for disti- nguishing between high amylose miaze and normal ones. We sequenced cDNAs from high-amylose and normal corn inbred lines and designed a co-dominant functional marker ae-InDel. Our result showed that the ae-InDel could distinguish aeae homozygous genotype from homozygous genotype AeAe and h... |
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| Keywords: | Miaze High-Amylose amylose-extender Functional marker |
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