| 农杆菌介导AVP1基因转化甜菜 |
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| 引用本文： | 王晓娇,孙亚卿,李国龙,温丽,张少英. 农杆菌介导AVP1基因转化甜菜[J]. 分子植物育种, 2011, 9(3): 370-375. DOI: 10.3969/mpb.009.000370 |
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| 作者姓名： | 王晓娇 孙亚卿 李国龙 温丽 张少英 |
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| 作者单位： | 内蒙古农业大学甜菜生理研究所; |
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| 基金项目： | 国家甜菜现代产业体系项目(nycytx-25-04)资助 |
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| 摘 要： | 甜菜是我国重要的糖料作物之一.为了提高甜菜含糖量和产量,增强其抗逆性,本研究应用植物基因工程手段对甜菜进行遗传改良.实验以甜菜DF1031品系为受体材料,利用农杆菌介导法将AVP1基因转入甜菜子叶节,获得转基因植株.研究了菌液侵染浓度、侵染时间、共培养时间、抗生素羧苄青霉素(Carb)、头孢霉素(Cef)以及筛选剂卡那...
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| 关 键 词： | 甜菜 液泡膜H+-PPase 农杆菌介导 遗传转化 |
Agrobacterium-mediated Transformation of Sugar Beet with Vacuolar H+-PPase (AVP1) Gene |
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| Wang Xiaojiao,Sun Yaqing,Li Guolong,Wen li,Zhang Shaoying. Agrobacterium-mediated Transformation of Sugar Beet with Vacuolar H+-PPase (AVP1) Gene[J]. Molecular Plant Breeding, 2011, 9(3): 370-375. DOI: 10.3969/mpb.009.000370 |
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| Authors: | Wang Xiaojiao Sun Yaqing Li Guolong Wen li Zhang Shaoying |
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| Affiliation: | Wang Xiaojiao Sun Yaqing Li Guolong Wen li Zhang Shaoying Sugar Beet Physiological Institute,Inner Mongolia Agricultural University,Hohhot,010018 |
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| Abstract: | Sugar beet is one of the important sugar crops. In order to improve the sugar content and yield of sugar beet, enhance its resistance, this study applies plant gene engineering means of sugar beet on genetic improvement. AVP1 gene was successfully transformed into the explants of cotyledon node. The study on effects of Agrobacterium concentration, immersed time, co-culture time, Carb, Cef and Kan on genetic transformation frequencies. An efficient genetic transformation system was established. The results s... |
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| Keywords: | Sugar beet (Beta Vulgaris) Vacuolar H -PPase Agrobacterium-mediated Genetic transformation |
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