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| 培育水稻恢复系抗稻褐飞虱基因导入系和聚合系 | |
| 引用本文： | 刘开雨,卢双楠,裘俊丽,李孝琼,马增凤,陈乔,李粲,韦素美,黄凤宽,张月雄,刘驰,黄大辉,李容柏.培育水稻恢复系抗稻褐飞虱基因导入系和聚合系[J].分子植物育种,2011,9(4):410-417. |
| 作者姓名： | 刘开雨 卢双楠 裘俊丽 李孝琼 马增凤 陈乔 李粲 韦素美 黄凤宽 张月雄 刘驰 黄大辉 李容柏 |
| 作者单位： | 1. 广西大学生命科学与技术学院,农学院,广西亚热带生物资源保护利用重点实验室,南宁,530004;广西农科院水稻研究所,微生物研究所,植物保护研究所,广西作物遗传改良生物技术重点开放实验室,南宁,530007 2. 广西大学生命科学与技术学院,农学院,广西亚热带生物资源保护利用重点实验室,南宁,530004 3. 广西农科院水稻研究所,微生物研究所,植物保护研究所,广西作物遗传改良生物技术重点开放实验室,南宁,530007 |
| 基金项目： | 国家自然科学基金(30960190); 国家863计划(2006AA10Z1C52006AA100101-22007AA0214052007AA-10Z191); 广西科学基金项目应用基础研究专项(桂科基0833025); 广西科学研究与技术开发计划(桂科能05112001-1A1桂科能0630006-5F桂科能0815011-6-109-007-08)共同资助 |
| 摘 要： | 稻褐飞虱是水稻的主要虫害之一,培育优良的抗性基因聚合系对于防治稻褐飞虱具有重要的意义.本研究通过回交、分子标记辅助选择和接虫鉴定三者相结合的办法,将抗稻褐飞虱基因Bph3和Bph24(t)分别导入主栽杂交水稻恢复系广恢998、9311、R15、明恢63、R29中,最终获得遗传稳定的Bph3导入系32份和Bph24(t)... |
| 关 键 词： | 稻褐飞虱 Bph3Bph24(t)聚合系 抗性基因导入系 BPHR96 恢复系 水稻育种 |
Development of Brown Planthopper Resistance Gene-Inserted and-Accumulated Lines of Rice Restorers |
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| Liu Kaiyu,Lu Shuangnan,Qiu Junli,Li Xiaoqiong,Ma Zengfeng,Chen Qiao,Li Can,Wei Sumei,Huang Fengkuan,Zhang Yuexiong,Liu Chi,Huang Dahui,Li Rongbai.Development of Brown Planthopper Resistance Gene-Inserted and-Accumulated Lines of Rice Restorers[J].Molecular Plant Breeding,2011,9(4):410-417. | |
| Authors: | Liu Kaiyu Lu Shuangnan Qiu Junli Li Xiaoqiong Ma Zengfeng Chen Qiao Li Can Wei Sumei Huang Fengkuan Zhang Yuexiong Liu Chi Huang Dahui Li Rongbai |
| Institution: | Liu Kaiyu 1,2 Lu Shuangnan 1,2 Qiu Junli 1 Li Xiaoqiong 2 Ma Zengfeng 2 Chen Qiao 1 Li Can 1,2 Wei Sumei 2 Huang Fengkuan 2 Zhang Yuexiong 2 Liu Chi 2 Huang Dahui 2 Li Rongbai 1,2 1 College of Life Science and Technology,College of Agriculture and State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Guangxi University,Nanning,530005,2 Institutes of Rice Research,Microbiology Research and Plant Protection,and Guangxi Crop Improvement and Biotechnology Lab,Guangxi Agricultur... |
| Abstract: | The Brown planthopper(BPH,Nilaparvata lugens(St?l)) is one of the main insect pests of rice.To breed resistance gene-accumulated lines is of the great importance for the control of the BPH.In this study,BPH resistance genes,Bph3 and Bph24(t),were transferred into 5 main restorer lines,Guanghui998,9311,R15,Minghui63 and R29,via backcross,molecular marker-assisted selection(MAS) and artificial infestation of insect.Thirty-two Bph3-inserted lines,22 Bph24(t)-inserted lines and 13 Bph3Bph24(t)-accumulated lines were developed.Results from artificial infestation of the BPH showed that the Bph3 and the Bph24(t)-inserted lines were moderately resistant or resistant to the BPH with mean resistance scores 3.96 and 3.84,respectively,while Bph3Bph24(t)-inserted lines showed the highest resistance with resistance scores from 2.38 to 2.65 and mean score 2.52.Resultsindicate that BPH resistance gene accumulation result in additive gene effect.SSR analysis showed that the recovery of genetic backgrounds of the receptors in the resistance gene-inserted lines and Bph3Bph24(t)-inserted lines were more than 90%.Analysis of agronomic traits revealed that there were no significant differences in most agronomic traits between most of the gene-accumulated lines and their corresponding receptor parents in plant height,flag leaf length and breadth,effective panicles and 1 000-grain weight.The above results preliminary demonstrated that the BPH resistance gene-inserted lines and accumulated-lines derived from 5 restorers of main commercial rice hybrids were successfully developed and these resistance-improved restorer lines were with high potential in developing new promising resistant rice hybrids.This work would also provide a solid foundation in breeding materials for the establishment of the rice resistant breeding platform. |
| Keywords: | Brown planthopper(BPH) Bph3Bph24(t)-accumulated line Resistance gene-inserted lines BPHR96 Restorer lines Rice breeding |
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