| 超量表达水稻miRNA167a调控株型的研究 |
| |
| 引用本文： | 韦懿,陈志辉,陈国兴,熊立仲,吴昌银.超量表达水稻miRNA167a调控株型的研究[J].分子植物育种,2011,9(4):390-396. |
| |
| 作者姓名： | 韦懿 陈志辉 陈国兴 熊立仲 吴昌银 |
| |
| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,华中农业大学国家植物基因研究中心,武汉,430070 |
| |
| 基金项目： | 国家转基因专项基因表达调控技术研究项目(2008ZX08010-002)资助 |
| |
| 摘 要： | 本研究将水稻MIR 167基因家族成员之一MIR167a基因构建到玉米Ubiquitin基因组成型启动子下游,采用农杆菌介导的遗传转化方法实现了miRNA 167a在水稻品种中花11号中的超量表达.Southern杂交进一步获得了单拷贝基因插入的转基因植株.Ubi::MIR 167a超量表达植株表现为矮化、分孽角度增大...
|
| 关 键 词： | 水稻 miRNA 167a 株型调控 |
Study of Overexpressing miRNA 167a to Regulate the Architecture in Oryza sativa |
| |
| Wei Yi,Chen Zhihui,Chen Guoxing,Xiong Lizhong,Wu Changyin.Study of Overexpressing miRNA 167a to Regulate the Architecture in Oryza sativa[J].Molecular Plant Breeding,2011,9(4):390-396. |
| |
| Authors: | Wei Yi Chen Zhihui Chen Guoxing Xiong Lizhong Wu Changyin |
| |
| Institution: | Wei Yi Chen Zhihui Chen Guoxing Xiong Lizhong Wu Changyin National Key Laboratory of Crop Genetic Improvement,National Center of Plant Gene Research,Huazhong Agricultural University,Wuhan,430070 |
| |
| Abstract: | To get the miRNA167a overexpressing transgenic rice plants,we constructed miRNA167a,one member of the MIR167 gene family,under the maize Ubiquitin promoter and transformed the vector into Zhonghua 11 by Agrobacterium-mediated transformation.We identified the single copy transgenic plant by Southern blotting.The miRNA167a overexpressing plants performed varieties of plant architecture changes,such as dwarf,increase in the till angles and decrease in the effective tiller numbers.Further molecular analyses sho... |
| |
| Keywords: | Rice(Oryza sativa L ) miRNA167a Plant architecture regulation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
