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| 小麦热激蛋白基因TaHSP70克隆及其在植物防卫和抗逆反应中的表达分析 | |
| 引用本文： | 安艳秋,蔺瑞明,冯晶,王凤涛,徐世昌,许玉凤. 小麦热激蛋白基因TaHSP70克隆及其在植物防卫和抗逆反应中的表达分析[J]. 分子植物育种, 2011, 9(4): 402-409. DOI: 10.3969/mpb.009.000402 |
| 作者姓名： | 安艳秋 蔺瑞明 冯晶 王凤涛 徐世昌 许玉凤 |
| 作者单位： | 1. 沈阳农业大学生物科学技术学院,沈阳,110866;中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193 2. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193 3. 沈阳农业大学生物科学技术学院,沈阳,110866 |
| 基金项目： | 转基因生物新品种培育重大专项(2009ZX08009-053B); 国家自然基金国际合作项目(30821140351); “973”项目(2011CB100403); 公益性行业专项(200903035)共同资助 |
| 摘 要： | 热激蛋白是一类结构非常保守的分子伴侣类型的应激蛋白,在调控动、植物代谢和信号传导等方面发挥重要作用.本研究利用同源克隆法,筛选非亲和条锈菌小种CYR32侵染诱导的cDNA文库,分离获得1个HSP70家族基因的全长cDNA序列TaHSP70.序列分析发现,该cDNA片段包含1个2 412 by开放阅读框,推测其编码包含6... |
| 关 键 词： | 小麦 HSP70 条锈菌 白粉菌 防卫反应 |
Moleular Cloning of Wheat Heat Shock Protein Gene TaHSP70 and Expression Analysis in Plant Defense and Stress Responses |
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| An Yanqiu,Lin Ruiming,Feng Jing,Wang Fengtao,Xu Shichang,Xu Yufeng. Moleular Cloning of Wheat Heat Shock Protein Gene TaHSP70 and Expression Analysis in Plant Defense and Stress Responses[J]. Molecular Plant Breeding, 2011, 9(4): 402-409. DOI: 10.3969/mpb.009.000402 | |
| Authors: | An Yanqiu Lin Ruiming Feng Jing Wang Fengtao Xu Shichang Xu Yufeng |
| Affiliation: | An Yanqiu 1,2 Lin Ruiming 2 Feng Jing 2 Wang Fengtao 2 Xu Shichang 2 Xu Yufeng 1 1 College of Biological Science and Technology,Shenyang Agricultural University,Shenyang,110866,2 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,100193 |
| Abstract: | Heat shock proteins are a kind of molecular chaperones,of which structure are highly conserved,and play essential roles in regulating various metabolic processes and molecular signal transductions in animals and plants.Through homologous PCR cloning method,a novel full-length cDNA encoding a HSP70 protein was obtained from a cDNA library of near-isogenic line Taichung 29*6/Yr5 inoculated with an incompatible race CYR32 of Puccinian striiformis,and was named as TaHSP70.Sequence analyses indicated that TaHSP7... |
| Keywords: | Wheat HSP70 Puccinia striiformis Erysiphe graminis Stress resistance |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |