| 小白鼠乳腺上皮细胞的体外培养 |
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| 引用本文： | 陶海静,王老七.小白鼠乳腺上皮细胞的体外培养[J].郑州牧业工程高等专科学校学报,2003,23(2):81-82,100. |
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| 作者姓名： | 陶海静 王老七 |
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| 作者单位： | 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011 |
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| 摘 要： | 本试验对小白鼠乳腺上皮细胞的原代培养方法进行了探索。胶原酶消化法和组织块种植法均可用于培养小白鼠原代乳腺细胞。胶原酶消化法容易得到均匀的乳腺细胞，且上皮细胞所占比例也比较高。组织块种植法不容易丢失、损伤上皮细胞，然而上皮细胞长出较晚，且占比例较低。结果表明，可结合组织块转移、胰蛋白酶消化纯化上皮细胞。
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| 关 键 词： | 小白鼠 乳腺 上皮细胞 体外培养 原代培养 胶原酶消化法 组织块种植法 胰蛋白酶 |
| 文章编号： | 1008-3111(2003)02-0081-02 |
In vitro culture of mammary epithelial cells in mice |
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| Institution: | (Biological Engineering Department of Zhengzhou College of Animal Husbandry & Engineering,Zhengzhou,Henan 450011,China) |
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| Abstract: | The methodology of primary culture of mammary epithelial cells in micewas studied in this experiment. Both collagenase digestion and tissue cultivation could be used in culturing mammary epithelial cells. By collagenase digestion, well-proportioned mammary cells were easily obtained with a higher percentageof epithelial cells. By tissue cultivation, epithelial cells were well kept andprotected, but grew out late with a lower percentage. It was shown that epithelial cells could be purified by both tissue transfer and collagenase digestion |
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| Keywords: | mouse mammary epithelial cell in vitro culture |
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