河套苹果梨离体组织在不同激素水平的生长及抗菌力分析

利用组织培养对内蒙古特色资源的河套苹果梨进行遗传保护、品质改良以及生物技术操作具有重要的理论意义和实践价值。试验利用NAA、2,4_D和6_BA对苹果梨叶片进行组织脱分化研究。结果表明,0~0.2 mg/L的NAA与0.1 mg/L的6_BA组合可有效促进叶片组织脱分化,形成愈伤组织或直接诱导叶芽的萌发。NAA与2,4_D组合未表现出有明显促进河套苹果梨叶片组织脱分化的作用。在叶片组织脱分化过程中,外植体的染菌率与激素水平存在一定的线性关系。

Analysis on Growth and Ability of Antimicrobial in Different Hormone of Hetao Apple-pear

In order to accomplish the research project of Hetao apple-pear's tissue culture,this study conducted a primary study of tissue dedifferentiation of Hetao apple-pear under the effects of the three plant hormones NAA,2,4-D and 6-BA.The results indicated that the effects of combination of NAA(0-0.2 mg/L) and 6-BA(0.1 mg/L) can effectively dedifferentiate the vegetative tissue(buds) of Hetao apple-pear,and stimulated callus formation or directed growth of leave buds.The combinations of NAA and 2,4-D had potential effects on tissue dedifferentiation of Hetao apple-pear.Meanwhile,it was found that the intensity of microorganism contamination of explants during tissue culturing were correlated to the concentration of plant hormone.