首页 | 本学科首页   官方微博 | 高级检索  
     


Detection of Anti-listeriolysin O and Listeria monocytogenes in Experimentally Infected Buffaloes (Bubalus bubalis)
Authors:Chaudhari  S.P.  Malik  S.V.S.  Banu Rekha  G.  Barbuddhe  S.B.
Affiliation:(1) Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, UP, India
Abstract:The kinetics of antibody production against listeriolysin O (ALLO) and the recovery pattern of Listeria monocytogenes from bacteriological samples were studied following oral infection of buffalo calves with 3×109 cells each of pathogenic L. monocytogenes. Antibodies to LLO appeared by 7–10 days post infection (PI), with a shallow peak between days 16 and 36 PI, when tested by indirect plate-ELISA. The titres of ALLO in all the animals then declined slowly but remained detectable up to day 70 PI. In dot-ELISA, ALLO could be detected by days 5 to 7 PI, and with higher titres than with the plate-ELISA. The pathogen was recovered at low rates as ALLO first appeared but was absent in the faecal, nasal and blood cultures as production of ALLO peaked.
Keywords:antibody  buffalo  diagnosis  ELISA  kinetics  Listeria monocytogenes  listeriolysin  serology
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号