牦牛不同妊娠阶段孕酮的主要来源器官研究

为明确牦牛不同妊娠阶段孕酮的来源,分别对53头不同妊娠阶段牦牛的黄体组织、肾上腺组织和胎盘组织进行体外细胞培养,同时对44头牦牛的上述组织进行匀浆处理,采用放射免疫分析法（RIA）测定培养液及组织匀浆上清液中孕酮的含量。结果显示,黄体细胞在体外培养时,妊娠前2个月内孕酮分泌量显著升高（P〈0．05）：妊娠2-5个月,培养液中孕酮含量变化不明显（P〉0．05）,但在妊娠5个月以后,其浓度又开始显著增加（P〈0．01）。肾上腺组织体外培养时,分泌孕酮的能力随不同个体差别较大,孕酮浓度在整个妊娠期变化不明显（P〉0．05）。胎盘组织体外培养时仅分泌少量孕酮,且在整个妊娠期其浓度变化不明显。黄体组织匀浆上清液中孕酮浓度在妊娠前2个月内呈上升趋势（P〈0．05）,之后其浓度开始下降,至妊娠第4个月时下降到最低水平,以后又逐渐上升（P〈0．05）,妊娠6-7个月时达到峰值,临近分娩时,又下降至较低水平。肾上腺组织匀浆中孕酮含量的变化差异较大,且无明显的变化趋势,胎盘组织匀浆中孕酮的含量也很低,甚至检测不到。结果表明,黄体是牦牛整个妊娠期孕酮的主要来源器官。

Studies on the source of progesterone during different period of gestation in yaks

In order to determine the source of progesterone during the wholg gestation in yaks,corpus luteum,adrenal and placental tissues were studied to make sure whether they are capable of progesterone biosynthesis in 97 pregnant yaks during different period of gestation.Progesterone was detected in either the media of cultured cells of the tissues.or in the tissue homogenate by radioimmunoassay（RIA）.The results showed that progesterone comcentrations in the media of cultured luteum cells in vitro increased gradually during the first two months of gestation（P〈0.05）.During the following three months,progesterone values changed slightly（P〉0.05）.Thereafter,the concentration of progesterone increased dramatically again after the fifth month of gestation（P〈0.01）.In the media of cultured adrenal cells,progesterone concentrations were relatively low,and had little changes during the whole period of gestation（P〉0.05）.The levels of progesterone in the media of cultured placental cells were either very low or non-detectable.During the first 2 months of gestation,progesterone concentrations in the tissue homogenate of corpus luteum increased gradually（P〈0.05）,whil decreased dramatically in the following 2 months（P〈0.05）,and increased again after the fourth month of gestation（P〈0.05）,reaching its peak value at month 6-7 of gestation;then decreased again.The concentrations of progesterone in the adrenal cortical tissue homogenates and the placental tissue homogenate were very low or non-detectable.These results suggested that the main source of progesterone during the period of gestation in yaks is corpus luteum.and it is necessary for yak to sustain pregnancy.