Genetics of self-compatibility in a self-incompatible wild diploid potato species Solanum chacoense. 2. Localization of an S locus inhibitor (Sli) gene on the potato genome using DNA markers |
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Authors: | Kazuyoshi Hosaka Robert E Hanneman Jr |
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Institution: | (1) Experimental Farm, Kobe University, 1348 Uzurano, Kasai, Hyogo 675-2103, Japan;(2) USDA, Agricultural Research Service, Vegetable Crops Research Unit, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, Wisconsin, 53706, U.S.A |
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Abstract: | A self-compatible (SC) hybrid plant F1-1 was obtained from a cross between a SC variant of a wild diploid potato species, Solanum chacoense, and a self-incompatible
(SI) cultivated diploid species, S. phureja. The clone F1-1 has previously been proposed to have a dominant S locus inhibitor gene (Sli) in a heterozygous condition. It was crossed
as a male parent with a selected clone from a S. stenotomum-S. phureja population, resulting in a segregating population consisting
of 116 hybrid plants. Self-compatibility was assessed by selfing each of the hybrids. Sixty-six of them were SC, while 35
were SI, showing a significant distortion from an expected Mendelian ratio of 1:1. A genetic linkage map was constructed using
DNA markers to localize the Sli gene. A total of 28 RAPD and 127 RFLP markers identified 109 mapping positions on 12 linkage
groups. The Sli gene was mapped at a distal end of chromosome 12. Since the S locus has been localized on chromosome 1 on
the potato RFLP map, it is confirmed that the Sli gene is independent of the S locus.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | genetic linkage map self-compatibility S locus inhibitor Sli gene Solanum chacoense |
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