草本威灵仙的组织培养与快速繁殖

 用种子切段作外植体，建立了草本威灵仙的组织培养和再生体系。试验结果表明：草本威灵仙种子切段诱导愈伤组织的最佳培养基为MS+6-BA 3 mg·L^-1 +NAA 0.3 mg·L^-1，诱导率达86.7％；附加6-BA 2 mg·L^-1+NAA 0．2 mg·L ^-1的Ms培养基有利于芽的分化和增殖，培养60 d左右，每个外植体分化形成的再生小植株数达到12～18个；在1/2 MS+IBA 0.5 mg·L^-1 的培养基中生根效果最好。移植时喷施营养液可以提高幼苗的成活率。

Tissue Culture and Rapid Propagation of Veronicastrum sibiricum (L.) Pennell

The explants from seed segments of Veronicastrum sibiricum (L．)Pennell were used to establish culture in vitro and regeneration system．The experiments showed that the best medium for callus induction from seed segm ents was MS with 3 mg·L^-1 6-BA and 0．3 mg·L^-1 NAA．Th e medium supplemented with 2 mg·L^-16-BA and 0.2 mg·L^-1 NAA was favorable to the diferentiation and proliferation of buds, and each explant regenerated 12-18 plantlets about 60 days．The growth of roots was best on 1/2 MS medium with supplement of IBA 0．5 mg·L^-1．