| 不同GO基因植物表达载体对番茄遗传转化效率的研究 |
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| 引用本文： | 王全华,李素梅,尹国香,曹守军,张焕春,李云. 不同GO基因植物表达载体对番茄遗传转化效率的研究[J]. 北方园艺, 2006, 0(6): 4-6 |
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| 作者姓名： | 王全华 李素梅 尹国香 曹守军 张焕春 李云 |
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| 作者单位： | 山东省烟台市农科院蔬菜所,265500;山东省烟台市福山区蔬菜办,265500 |
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| 基金项目： | 山东省农业良种产业化工程项目 |
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| 摘 要： | 通过PPT质量浓度的梯度试验,筛选出番茄子叶出愈、芽分化和再生植株生根的最适宜PPT浓度均为1.0 mg/L.pCPGO和pCBGO两个植物表达载体转化8个番茄自交系材料的试验结果表明,不同番茄自交系对这两种表达栽体的遗传转化效率存在较大的差异;相同的载体转化不同的番茄自交系,其遗传转化率也存在较大的差异;同一番茄品系,由于转化载体不同,遗传转化率也不同.pCPGO和pCBGO转化率最高的分别为Pr5 76.47%和Pr1 72.4%;而低的分别仅为Pr3 13.89%和Pr4 2.7%.
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| 关 键 词： | 番茄 GO植物表达载体 遗传转化 |
| 文章编号： | 1001-0009（2006）06-0004-03 |
| 收稿时间： | 2006-08-10 |
| 修稿时间： | 2006-08-10 |
Transformation Rate of Two Plant Expression Vector pCPGO and pCBGO with GO Gene in Tomato |
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| WANG Quan-hua,LI Su-mei,YIN Guo-xiang,CAO Shou-jun,ZHANG Huan-chun,LI Yun. Transformation Rate of Two Plant Expression Vector pCPGO and pCBGO with GO Gene in Tomato[J]. Northern Horticulture, 2006, 0(6): 4-6 |
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| Authors: | WANG Quan-hua LI Su-mei YIN Guo-xiang CAO Shou-jun ZHANG Huan-chun LI Yun |
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| Affiliation: | 1. Yantai Academy of Agricultural Sciences, Shandong, 265500 ; 2. Yantai Fushan District Vegetable Office, Shandong, 265500 |
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| Abstract: | Four concentration of PPT was tested in tomato.Results showed the optimistic PPT concentration of calus,shoot formation and rootage of tomato explants were 1.0 mg/L.Eight tomato lines were transformed using plant expression vector pCPGO and pCBGO.The results demonstrated transformation frequency of various tomato lines was roughly different in two expression vector.That was in same vector and transformation frequency of same tomato line duing to various plant expression vector.The highest transformation frequency of pCPGO and pCBGO in all lines were Pr5,76.47% and Pr1,72.4%,the lowest of those were Pr3,13.89% and Pr4,2.7%. |
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| Keywords: | Tomato GO plant expression vector Transformation |
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