| 鸡免疫球蛋白MFc(IgMFc)重链的分离纯化及其抗血清的制备 |
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| 引用本文： | 李庆章,刘忠贵.鸡免疫球蛋白MFc(IgMFc)重链的分离纯化及其抗血清的制备[J].东北农业大学学报,1991(3). |
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| 作者姓名： | 李庆章 刘忠贵 |
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| 作者单位： | 东北农学院兽医病理寄生虫学教研室，东北农学院兽医病理寄生虫学教研室 |
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| 摘 要： | 经硫酸铵盐析沉淀并经 DEAE_(32)—纤维素枉色谱分离粗制 IgM,再经聚丙烯酰胺凝胶电泳(PAGE)进一步分离纯化鸡血清 IgM。然后用木瓜蛋白酶水解IgM,经 DEAE_(32)—纤维素柱色谱纯化 IgMFc 重链,再用 IgMFc 重链免疫家兔制取兔抗鸡 IgMFc 重链抗血清。
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| 关 键 词： | 鸡 IgM IgMFc重链 抗血清 |
ISOLATION AND PURIFICATION OF CHICKEN IMMUNOGLOBULIN MIFc(IgMFc)HEAVY CHAIN AND PRE PARATION OF ITS ANTISERA |
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| Li Qingzhang Liu Zhonggui.ISOLATION AND PURIFICATION OF CHICKEN IMMUNOGLOBULIN MIFc(IgMFc)HEAVY CHAIN AND PRE PARATION OF ITS ANTISERA[J].Journal of Northeast Agricultural University,1991(3). |
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| Authors: | Li Qingzhang Liu Zhonggui |
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| Abstract: | Rough immunoglobulin M (IgM) in chicken serum was obtained by salting-out withammonium sulfate and DEAT_(32)-cellulose column chromatohgraphy,The IgM was furtherisolated and purified by polyacrylamide gel electrophoresis (PAGE).Then papain was usedto hydrolize the pure IgM and the IgMFc heavy chains were purified by DEST_(32)—cellulosecolumn chromatography.The rabbit anti-chicken IgMFc heavy chain antisra wer preparedafter the rabbits were injected with the IgMFc heavy chain. |
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| Keywords: | chicken IgM IgMFc heavy chain antiserum |
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