Cu2+对红豆杉培养细胞中紫杉醇形成的影响

用Ｃｕ＾２＋对红豆杉（Ｔａｘｕｓ ｃｈｉｎｅｎｓｉｓ）培养细胞中紫杉醇形成的影响进行了研究。在红豆杉细胞悬浮培养２０ｄ即指数生长期末，每１Ｌ细胞悬浮培养物中加入３０μｍｏｌ ＣｕＣｌ２，Ｃｕ＾２＋促进紫杉醇形成的作用最大。添加ＣｕＣｌ２对红豆杉细胞生长没有明显影响，但引起培养细胞中可溶性蛋白质含量、苯丙氨酸解氮酶活性及培养基ｐＨ值的变化。

EFFECTS OF Cu2+ ON TAXOL FORMATION IN CELL CULTURES OF Tax us chinensis

The promotion of taxol formation by Cu2 is described in this paper. The optimum improvement of taxol production by Cu2 was got when 30 mol/L CuCl2 cell suspension cultures was added on the 20th day after inoculation, namely, the end of exponential growth phase. The cell growth was not significantly affected by the adding, but the intracellular soluble protein content and phenylalanine amonialyase activity of cell suspension cultures, and the medium pH value changed.;