玻璃化冷冻对小鼠GV期卵母细胞发育能力的影响

试验首次采用OPS法玻璃化冷冻小鼠GV期卵母细胞(不带卵丘细胞,下同),同时尝试用EDFS30对小鼠卵巢进行细管法玻璃化冷冻,以研究GV期卵母细胞冷冻后的发育潜力。首先,利用MEM培养和MEM-腔前卵泡培养新鲜GV期卵母细胞,并把较好的培养方式用于冷冻后培养试验。2种培养方式培养24h后新鲜GV期卵母细胞成熟率无显著性差异;OPS法冷冻的GV期卵母细胞解冻后成熟率及体外受精后卵裂率与对照组差异不显著(P>0.05)。细管法冷冻卵巢组织的GV期卵母细胞成熟率极显著低于对照组(P<0.01),其受精后未获得受精卵。结果表明:OPS法可有效地冷冻保存小鼠GV期卵母细胞,而细管法冷冻小鼠卵巢对GV期卵母细胞损伤较大。

Effects of Vitrification on the Development of Mouse GV-oocytes

To investigate the potential development of thawed GV-oocytes,the mouse GV-occytes(without comulus cells) were vitrified using OPS method and the mouse ovary was vitrified by straw method employing EDFS30 as vitrification solution for the first time.At first,in order to choose the optimal culture system for GV-oocytes after vitrification,the fresh GV-oocytes were cultured in MEM or MEM-preantral follicle.The result showed that there was no significant difference between the two culture methods;the rates of maturation or the rates of cleavage did not differ between the fresh and vitrified group(P > 0.05).However,the GV-oocytes separated from the vitrified ovary showed significant difference(P < 0.01) with the control in the rates of maturation,and no zygote was gained in the present study.In Conclusion,GV-oocytes can be cryopreserved efficiently by OPS method,while the GV-oocytes derived from vitrified ovary showed limited potential viability.