烟草漂浮育苗旧苗盘残留物TMV带毒检测

为了建立烟草漂浮育苗旧苗盘残留物中烟草花叶病毒TMV的检测方法，以TMV病苗干根为材料，采用直接研磨法、液氮研磨法和浸泡研磨法制样，效果分别用三抗体夹心法（TAS—ELISA），TMV试纸条和枯斑寄主法检测灵敏度．结果表明，3种方法制备的样品都能用TAS-ELISA和试纸条检测出阳性，且3种制样方法间的差异小；3种制样方法制备的样品都具有侵染力，浸泡研磨法最强，直接研磨法次之，液氮研磨法最弱．TAS—ELISA，TMV试纸条和枯斑寄主接种的检测下限分别为千病根占健康根质量的0．01％，0．10％和0．10％．

Detection of TMV in Residue of Tobacco Roots on Tray Using in Float Seedling System

In order to develop detection methods of Tobacco mosaic virus in residue of tobacco roots on tray using in float seedling system,by using dry roots of TMV-infected tobacco float seedling,three sample prepare methods including direct grinding,liquid nitrogen grinding and dip grinding,and three detection methods sensitivity including TAS-ELISA,TMV-Imunostrip and local lesion assay are evaluated.Samples prepared by three methods can detect TMV positive and show no obvious difference between each other when detected by TAS-ELISA and TMV-Imunostrip,Samples prepared by three methods show TMV effective when inoculated into Nicotiana glutinosa,the local lesion number order is: dip grinding >direct grinding>liquid nitrogen grinding.The detection threshold of TAS-ELISA,TMV-Imunostrip and local lesion assay are 0.01%,0.10%,0.10% dry TMV-infected roots among healthy roots respectively.