| 一种适于SSR-PCR的大豆叶片微量DNA简便提取方法 |
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| 引用本文： | 谢皓,陈学珍,冯永庆,常娥,方莹莹,王鲁,张绍光. 一种适于SSR-PCR的大豆叶片微量DNA简便提取方法[J]. 北京农学院学报, 2007, 22(1): 62-64 |
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| 作者姓名： | 谢皓 陈学珍 冯永庆 常娥 方莹莹 王鲁 张绍光 |
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| 作者单位： | 北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206 |
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| 摘 要： | 基于PCR技术的SSR标记已经广泛用于基因鉴定、遗传图谱和分子标记辅助育种。为了建立一种适于SSR-PCR的DNA快速提取方法,利用一种新的磨样技巧和减少DNA提取步骤的方法,以大豆叶片为样品对传统CTAB法进行改进。结果表明:新的提取方法与传统CTAB法相比,具有提取速度快、试剂费用低和不需在液氮中磨样等优势。提取的DNA可用于大豆SSR-PCR分析。
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| 关 键 词： | DNA 提取方法 大豆 |
| 文章编号： | 1002-3186(2007)01-0062-03 |
| 修稿时间： | 2006-11-09 |
A Easy Method of Extracting Tiny DNA from the Soybean Leaves for SSR-PCR |
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| XIE Hao,CHEN Xue-zhen,FENG Yong-qing,CHANG E,FANG Ying-ying,WANG Lu,ZHANG Shao-guang. A Easy Method of Extracting Tiny DNA from the Soybean Leaves for SSR-PCR[J]. Journal of Beijing Agricultural College, 2007, 22(1): 62-64 |
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| Authors: | XIE Hao CHEN Xue-zhen FENG Yong-qing CHANG E FANG Ying-ying WANG Lu ZHANG Shao-guang |
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| Affiliation: | Department of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206,China |
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| Abstract: | SSR marker,a PCR-based marker,has been widely used to identify and tag gene as well as in marker assisted selection.To develop a faster DNA extraction method for SSR-PCR,the conventional CTAB method was improved by a novel pulverizing technique to simplify extractive process in soybean leaves.The results showed that new extraction had advantages of being faster,less expensive and no requiring liquid nitrogen to pulverize the samples than conventional CTAB method,and the DNA derived from new method could be used to SSRPCR analysis in soybean. |
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| Keywords: | DNA extraction Soybean |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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