Molecular cloning of insulin-like growth factor-I complimentary DNA and promoter region in the domestic duck (Anas platyrhynchos)* |
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| Authors: | Norio KANSAKU Eriko YAGI Asako NAKADA Hisato OKABAYASHI Daniel GUEMENE David ZADWORNY |
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| Affiliation: | Laboratory of Animal Genetics and Breeding, Azabu University, Sagamihara-shi, Japan,; INRA-SRA, Nouzilly, France;and Department of Animal Science, McGill University, Ste Anne de Bellevue, Quebec, Canada |
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| Abstract: | Complementary DNA (cDNA) and the flanking region of insulin‐like growth factor‐I (IGF‐I) of domesticated duck were cloned. The nucleotide sequence analysis of the cDNA showed seven and eight bases different, respectively, from chicken and turkey IGF‐I cDNA within the coding region. The amino acid sequence of prepro IGF‐I differed by one and two amino acids from those observed in chicken and turkey, respectively. However, no amino acid substitution was observed in the mature IGF‐I region. Sequence analysis of the promoter region and exon 1 of the duck IGF‐I revealed a high degree of similarity to that of the chicken IGF‐I gene. These results suggest that the mechanisms which regulate expression of the IGF‐I gene may be widely conserved in avian species. |
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| Keywords: | duck insulin-like growth factor-I messenger ribonucleic acid promoter |
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