| 转TPSP融合基因小麦植株的获得及抗旱性初步鉴定 |
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| 引用本文： | 李永春,王潇,陈焕丽,孟凡荣,陈雷,尹钧. 转TPSP融合基因小麦植株的获得及抗旱性初步鉴定[J]. 麦类作物学报, 2009, 29(2) |
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| 作者姓名： | 李永春 王潇 陈焕丽 孟凡荣 陈雷 尹钧 |
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| 作者单位： | 1. 河南农业大学/国家小麦工程技术研究中心,河南郑州,450002
2. 河南农业大学生命科学学院,河南郑州,450002 |
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| 基金项目： | 河南省重点科拉攻关计划农业项目 |
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| 摘 要： | 为了培育抗旱小麦新材料,在构建舍大肠杆茵海藻糖-6-磷酸合成酶基因(otsA)和海藻糖-6磷酸磷酸酯酶基因(otsB)融合基因TPSP的表达栽体pBS-ATPSP的基础上.利用花粉管通道法对小麦品种豫麦34和豫麦18进行了遗传转化.两个品种共处理2 495朵小花,获得T0代种子1 611粒.两个品种分别获得T1代小麦植株857和659株,经PCR鉴定转基因植株分别为11株和7株.通过半定量RT-PCR技术对4个T2代转基因株系中融合基因TPSP的表达分析显示,3个株系中TPSP基因受水分胁迫而诱导表达,其中2个株系在温室进行的干旱胁迫鉴定中表现出较强的抗旱能力.
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| 关 键 词： | 小麦 TPSP融合基因 抗旱性 |
Obtaining of Transgenic Wheat Plants with TPSP Fusion Gene and Preliminary Assay of Drought Tolerance |
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| LI Yong-chun,WANG Xiao,CHEN Huan-li,MENG Fan-rong,CHEN Lei,YIN Jun. Obtaining of Transgenic Wheat Plants with TPSP Fusion Gene and Preliminary Assay of Drought Tolerance[J]. Journal of Triticeae Crops, 2009, 29(2) |
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| Authors: | LI Yong-chun WANG Xiao CHEN Huan-li MENG Fan-rong CHEN Lei YIN Jun |
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| Affiliation: | 1.National Engineering Researching Center for Wheat;Henan Agricultural University;Zhengzhou;Henan 450002;China;2.College of Life Science;China |
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| Abstract: | In order to obtain drought resistant wheat materials based on the expression vector pBS-ATPSP harboring a fusion gene TPSP(fused by the otsA and otsB gene which encodes trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase respectively),two wheat cultivars,Yumai 34 and Yumai 18,were transformed via pollen tube pathway.In total,2 495 florets were treated with plasmid DNA and 1 611 T0 seeds were harvested,857 and 659 T1 plantlets were obtained from Yumai 34 and Yumai 18,respectively,and the PCR... |
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| Keywords: | Wheat TPSP fusion gene Drought tolerance |
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