蓖麻种质资源多样性AP-PCR与RMAPD分析

为了研究国内主要商业蓖麻品种的种质资源多样性,采用AP-PCR与RMAPD分子标记方法,对来自国内不同地区的31份蓖麻品种进行分析。AP-PCR分子标记的研究结果表明,9条引物扩增的条带为5~12条,共计82条带,平均每条引物扩增出9.1条带,扩增的多态性条带为2~10条,多态率为40%~90.91%,共计53条带,平均每条引物扩增出5.89条多态性带。在9条引物中,引物S3扩增出多态性条带最高,多态率达90.91%。蓖麻种质资源的RMAPD分析结果表明,84对引物扩增的条带为5~12条,共计85条带,平均每对引物扩增出8.5条带,扩增的多态性条带为3~10条,共计56条带,平均每条引物扩增出5.6条多态性带。在84对引物中,引物A3+B4扩增出的条带最多,多态性最高,多态率达83.3%。对AP-PCR与RMAPD分子标记的数据进行联合聚类分析表明,在遗传系数为0.56处,31份蓖麻种质被分成3大类群。2种分子标记之间的相关系数不显著(R=0.009 3)。

Castor Germplasm Diversity Analysis Using AP-PCR and RMAPD

In this paper,AP-PCR and RMAPD methods have been used to analyze 31 different kinds of Ricinus communis from different domestic regions.Results of AP-PCR show that the number of bands amplified from 9 primers ranges between 5 to 12,with an average of 9.1 bands by each primer and in a total number of 82 bands.The number of amplified polymorphic bands is between 2 to 10,with the polymorphism rate from 40% to 90.91%.5.89 polymorphic bands on average are amplified by each primer with a total of 53 bands.Among these 9 primers,primer S3 has the highest polymorphism with the polymorphism rate 90.91%.Results of RMAPD show that the number of bands amplified by 84 pairs of primers ranges from 5 to 12,with an average of 8.5 and in total of 85;the number of polymorphic bands ranges from 3 to 10,with an average of 5.6 and in total of 56.Among 84 pairs of primers,A3+B4 has the largest number and the highest polymorphism with the polymorphism rate 83.3%.The joint cluster analysis of AP-PCR and RMAPD suggests that 31 copies of Ricinus communis germplasm are divided into 3 categories in the section when the heritability coefficient is 0.56.The correlation coefficient of these two molecular marker methods is not significant(R=0.009 3).