| 辣椒粉中罗丹明B液相色谱检测方法的研究 |
| |
| 引用本文： | 林清荷,陈鑫,吴忠兴.辣椒粉中罗丹明B液相色谱检测方法的研究[J].农产品加工．学刊,2012(11):157-158,164. |
| |
| 作者姓名： | 林清荷 陈鑫 吴忠兴 |
| |
| 作者单位： | 福州市产品质量检验所 |
| |
| 摘 要： | 研究了辣椒粉中罗丹明B的液相色谱检测方法。试样中的罗丹明B用体积分数80%甲醇提取,液相色谱—荧光检测器测定,外标峰面积法定量。罗丹明B在5～500 ng/mL呈良好的线性关系,3个水平(10.0,30.0,50.0μg/kg)添加罗丹明B的回收率为88.7%～109.0%,相对标准偏差(RSD)为4.3%～5.7%,检出限为5μg/kg。此方法简便、快捷,适用于辣椒粉中罗丹明B的检测。
|
| 关 键 词： | 罗丹明B 高效液相色谱 荧光检测器 辣椒粉 |
HPLC Determination of Rhodamine B in Chilli Powder |
| |
| LIN Qing-he,CHEN Xin,WU Zhong-xing.HPLC Determination of Rhodamine B in Chilli Powder[J].Nongchanpin Jlagong.Xuekan,2012(11):157-158,164. |
| |
| Authors: | LIN Qing-he CHEN Xin WU Zhong-xing |
| |
| Institution: | (Fuzhou Product Quality Monitoring Bureau,Fuzhou,Fujian 350025,China) |
| |
| Abstract: | HPLC determination of rhodamine B in chilli powder is researched in this paper.Rhodamine B in sample is extracted by 80% methanol,HPLC-FLD analysis,and quantization by extemal standard method.The developed method shows a good linearity over the range of 5~100 ng/mL,average recoveries of rhodamine B in spiked samples at three levels(10.0,30.0,50.0 μg/kg) varied from 88.7% to 109.0%,with relative standard deviations between 4.3% and 5.7%.The limit of detection is 5 μg/kg.Therefore,this method,thanks to its simplicity and rapidity,has a good applicability to the determination of rhodamine B residue in chilli powder. |
| |
| Keywords: | rhodamine B HPLC FLD chilli powder |
| 本文献已被 CNKI 等数据库收录！ |
|
