| 棉花黄萎病拮抗细菌7-30菌株的筛选与鉴定(英文) |
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| 引用本文： | 雷白时,姜军坡,王伟,张冬冬,王全,朱宝成. 棉花黄萎病拮抗细菌7-30菌株的筛选与鉴定(英文)[J]. 农业科学与技术, 2008, 9(6): 127-131 |
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| 作者姓名： | 雷白时 姜军坡 王伟 张冬冬 王全 朱宝成 |
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| 作者单位： | 河北农业大学生命科学学院,河北保定,071001 |
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| 摘 要： | [Objective]The aim of this study was to screen the antagonistic spore-forming bacteria of Verticillium dahliae and identify its physiological and biochemical characteristics.[Method]Taking the cotton verticillium wilt pathogen Verticillium dahliae V-190 as the test microorganism,the antagonistic spore-forming bacteria were screened.Through the preliminary screening and secondary screening,an antagonistic bacteria strain 7-30 with stronger antibacterial activity was obtained,and its morphological characteristics,physiological and biochemical characteristics were also identified.[Result]84 antagonistic bacteria strains were isolated from soil in various places by the preliminary screening.Especially,18 strains with better antagonistic ability were screened again,so an antagonistic bacteria strain 7-30 with the diameter of inhibition zone 18.9 mm and stronger antibacterial activity was obtained.According to its morphological characteristics,physiological and biochemical characteristics,the antagonistic bacteria strain 7-30 was identified as Bacillus subtilis primarily.[Conclusion]The strain 7-30 was obtained as the antagonistic spore-forming bacteria of Verticillium dahliae.
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| 关 键 词： | Verticillium dahliae Antagonistic bacteria Screening Identification Bacillus subtilis |
Screening and Identification of Cotton Verticillium Wilt Antagonistic Bacteria Strain 7-30 |
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| LEI Bai-shi,JIANG Jun-po,WANG Wei,ZHANG Dong-dong,WANG Quan,ZHU Bao-cheng. Screening and Identification of Cotton Verticillium Wilt Antagonistic Bacteria Strain 7-30[J]. Agricultural Science ＆ Technology, 2008, 9(6): 127-131 |
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| Authors: | LEI Bai-shi JIANG Jun-po WANG Wei ZHANG Dong-dong WANG Quan ZHU Bao-cheng |
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| Affiliation: | College of Life Science,Agricultural University of Hebei,Baoding 071001 |
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| Abstract: | [Objective]The aim of this study was to screen the antagonistic spore-forming bacteria of Verticillium dahliae and identify its physiological and biochemical characteristics. [Method]Taking the cotton verticillium wilt pathogen Verticillium dahliae V-190 as the test microorganism, the antagonistic spore-forming bacteria were screened. Through the preliminary screening and secondary screening, an antagonistic bacteria strain 7-30 with stronger antibacterial activity was obtained, and its morphological characteristics, physiological and biochemical characteristics were also identified. [Result]84 antagonistic bacteria strains were isolated from soil in various places by the preliminary screening. Especially, 18 strains with better antagonistic ability were screened again, so an antagonistic bacteria strain 7-30 with the diameter of inhibition zone 18.9 mm and stronger antibacterial activity was obtained. According to its morphological characteristics, physiological and biochemical characteristics, the antagonistic bacteria strain 7-30 was identified as Bacillus subtilis primarily. [Conclusion]The strain 7-30 was obtained as the antagonistic spore-forming bacteria of Verticillium dahliae. |
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| Keywords: | Verticillium dahliae Antagonistic bacteria Screening Identification Bacillus subtilis |
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