| 饲料中硝呋烯腙的测定——高效液相色谱法 |
| |
| 引用本文： | 谭美英,易建希,武深树,陈福华,隆雪明,李裕. 饲料中硝呋烯腙的测定——高效液相色谱法[J]. 饲料工业, 2012, 33(9): 50-52 |
| |
| 作者姓名： | 谭美英 易建希 武深树 陈福华 隆雪明 李裕 |
| |
| 作者单位： | 湖南省兽药饲料监察所,湖南长沙,410006 |
| |
| 摘 要： | 试验建立了饲料中硝呋烯腙的高效液相色谱测定方法。饲料中的硝呋烯腙用二甲基亚砜-乙腈溶液提取,混合型阳离子固相萃取柱净化,庚烷磺酸钠、柠檬酸缓冲液+乙腈=55+45作为流动相,C18色谱柱,检测波长382 nm。结果表明,在0.05~50μg/ml浓度范围内线性关系良好,r=0.999 9。该方法的定量限为0.05 mg/kg。本法操作简单,结果准确,可用于饲料中硝呋烯腙的测定。
|
| 关 键 词： | 硝呋烯腙 饲料 高效液相色谱法 |
Determination of nitrovin in feed by HPLC |
| |
| Tan Meiying , Yi Jianxi , Wu Shenshu , Chen Fuhua , Long Xueming , Li Yu. Determination of nitrovin in feed by HPLC[J]. Feed Industry, 2012, 33(9): 50-52 |
| |
| Authors: | Tan Meiying Yi Jianxi Wu Shenshu Chen Fuhua Long Xueming Li Yu |
| |
| Affiliation: | Tan Meiying,Yi Jianxi,Wu Shenshu,Chen Fuhua,Long Xueming,Li Yu |
| |
| Abstract: | A method was established for determining nitrovin in feed by high performance liquid chromatography(HPLC).Nitrovin was extracted from feed by means of a solvent mixture consisting of dimethyl sulfoxide and acetonitrile solution,and purified by mixed cation SPE cartridge.It was performed using sodium heptanesulfonate and citrate buffer along with acetonitrile as mobile phase,in the proportion of 55:45.The separation was done on a C18 column and it was detected at 382 nm wavelength.The method provided good linearity from 0.05 μg/ml to 50 μg/ml(r=0.999 9),and the quantification limits was 0.05 mg/kg.It was a simple and accurate method for determination of nitrovin in feed. |
| |
| Keywords: | nitrovin feed high performance liquid chromatography(HPLC) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
