| 匍匐翦股颖转化受体系统的建立(简报) |
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| 引用本文： | 陈智勇,易自力,蒋建雄,周朴华,苏晶,储成才. 匍匐翦股颖转化受体系统的建立(简报)[J]. 草业学报, 2006, 15(6): 112-114 |
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| 作者姓名： | 陈智勇 易自力 蒋建雄 周朴华 苏晶 储成才 |
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| 作者单位： | 湖南农业大学生命与科学技术学院,湖南,长沙,410128;中国科学院遗传与发育生物学研究所,北京,100101 |
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| 基金项目： | 湖南省自然科学基金 , 湖南省教育厅科研基金 |
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| 摘 要： | 以回旋、潘那A-1、L-93三种匍匐翦股颖品种为材料,进行了离体再生培养和遗传转化的研究,建立了这些品种的胚性愈伤组织高频诱导与再生系统,确定了遗传转化过程中常用抗生素的临界浓度,并从外植体的选择、最适培养基及最佳分化时间等3个方面探讨了建立匍匐翦股颖转化受体系统的必要条件。
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| 关 键 词： | 匍匐翦股颖 胚性愈伤组织 转化受体系统 |
| 文章编号： | 1004-5759（2006）06-0112-03 |
| 收稿时间： | 2005-06-30 |
| 修稿时间： | 2005-06-30 |
Establishment of a transformation receptor system for Agrostis stolonifera |
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| CHEN Zhi-yong,YI Zi-li,JIANG Jian-xiong,ZHOU Pu-hua,SU Jing,CHU Cheng-cai. Establishment of a transformation receptor system for Agrostis stolonifera[J]. Acta Prataculturae Sinica, 2006, 15(6): 112-114 |
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| Authors: | CHEN Zhi-yong YI Zi-li JIANG Jian-xiong ZHOU Pu-hua SU Jing CHU Cheng-cai |
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| Affiliation: | 1. Hunan Agricultural University, Changsha 410128, China; 2. Institute of Genetics and Developmented Biology Chinese Academy of Science, Beijing 100101, China |
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| Abstract: | Mature seeds of three species of Lolium were used to develop an efficient regeneration and transformation system from embryonic calli of these species.The concentrations of different antibiotics for callus growth were confirmed.The essential requirement of establishing the transformation receptor system for Agrostis stolonifera was discussed from choosing plant tissues,confirming the most effective media,and selecting the best regeneration time. |
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| Keywords: | Agrostis stolonifera embryogenic callus transformation receptor system |
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