间接Dot－ELISA检测猪细小病毒抗原的研究

本文成功地建立了间接斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）用于检测ＰＰＶ抗原对纯化ＰＰＶ抗原的最低检出量为２．５ｎｇ／点。ＰＰＶ阳性猪血清的特异性阻断试验及交叉反应试验证明，该方法对ＰＰＶ抗原的检测具有特异性。以该方法检测ＰＰＶ人工感染兔样本和自然染猪样本，ＰＰＶ抗原的阳性检出率分别为肾样本１００％（１７／１７）、８１．８２％（９１／１１），肝样本１００％（１６／１６）、５６．５２％（１３／２３）。２０份随机样本的间接Ｄｏｔ－ＥＬＩＳＡ检测结果与病毒分离和鉴定结果相符。

Studies on the Detection of PPV Antigen Using Indirect Dot-ELISA

It's the first time that a standardized procedure of indirect dot enzyme-linked immunosorbentassay(Dot-ELISA)has been established for the detection of PPV antigen in this study.2.5ng per dot wasthe least of purified PPV antigen which the method could detect under the optimal condition. Its specificity indetection of PPV antigen was well shown by specific blockage tests of PPV positive porcine serum and thecrossreaction tests.The positive rates of PPV antigen of the experimentally infected rabbit specimens andfield porcine specimens measured by indirect DOt-ELISA were separately kidney specimens 100%(17/17)and81.8%(9/11),liver specimens 100%(16/16)and 55.52%(13/23).Results of detecition of 20-specimens bythe indirect Dot-ELISA were consistent with those of virus isolation and identification.