核苷类抗乙肝新药对孕鼠的生殖毒性影响研究

对不同试验组怀孕母鼠,在怀孕第6～15天每天通过尾静脉注射不同剂量的核苷类似物溶液(分别为大鼠有效剂量的40、20、10倍).怀孕第20天进行剖检,记录怀孕子宫质量、黄体数、早、晚期吸收胎数、活胎数和死胎数;活胎仔判断性别、称量质量后进行外观畸形和变异检查,以确定该供试品对SD大鼠母体、胚胎-胎仔发育和致畸性的影响.结果表明,核苷类似物供试品低、中剂量组孕鼠的体质量增长量、早晚期吸收胎数、窝平均活胎数、胎盘平均质量与对照组相比差异不显著(P>0.05),高剂量组的孕鼠的体质量增长量、胎仔死胎数,有死胎的母鼠数与对照组相比差异显著(P<0.05).

Study on Reproduction Toxicity Influence of Nucleoside Analogs to Pregnant Rats

The goal of this experiment is determine the female rats in the first 6-15 days of pregnancy tail intravenous injection of nucleoside analogues tested products on the mother,embryo-fetal development and the impact of teratogeny.After mating,the pregnant and healthy rats were randomly divided into high,medium,low and the control group.Then examination when pregnancy 20 days,recording each animal pregnant uterine weight,corpus luteum,the number of implantation,early and late absorption fetal,the numbers of live births and stillbirths; live births aberdeen judgment gender,weight,the appearance of malformations and conduct inspection variability.Through experimentation,observation of nucleoside analogues tested product slow-dose group of pregnant rats the size of the increase in body weight,early and late fetal absorption few,waterloo average number of live births,placental weight were compared with the control group had no significant differences between the high dose group of pregnant rats the size of the increase in body weight,litter number of stillbirths,the stillbirth of a few mice compared with the control group had significant differences.Note dose 200 mg·(kg·d)^-1 (effective dose in rats 40 times) will have significant toxicity in pregnant rats.The maternal toxicity,but no embryo red role and teratogenic effect on fetal development without adverse reaction.Therefore when use the drugs,its recommend dose is 100 mg·(kg·d) ^-1.