萝卜叶片和肉质根硫代葡萄糖苷组分与含量分析

采用HPLC分析法对‘滁州小五缨’、‘古岭夏萝卜’和‘云南白萝卜’3个萝卜品种叶片和肉质根的硫苷进行了分析。结果表明,3个地方品种叶片和肉质根硫苷组分相同,均含6种脂肪族硫苷和3种吲哚族硫苷,但品种及器官之间的硫苷含量存在显著差异。3个品种肉质根总硫苷含量分别为22.59,12.44和27.94 mg/g DM,其中95%以上是脂肪族硫苷。3个品种肉质根总硫苷与总脂肪族硫苷含量分别是叶片2.6～4.9倍与2.8～6.0倍；‘滁州小五缨’肉质根总吲哚族硫苷含量是叶片17倍,而‘古岭夏萝卜’和‘云南白萝卜’却只有0.37和0.77倍。4-甲基硫代-3-丁烯基硫苷是肉质根和叶片主要硫苷组分,相对含量分别为90.43%～93.92%和62.42%～74.05%。结果表明萝卜肉质根和叶片都含有丰富的硫苷,且不同品种以及器官之间的硫苷含量差异显著。

Composition and content of glucosinolates in leaves and edible roots of radish

The objective of this study was to evaluate the composition and content of GSs in leaves and edible roots of three local radish varieties by high-performance liquid chromatography. The results showed that the composition of GSs in leaves were the same as those in roots and included six aliphatic GSs and three indolyl GSs. However, there were significant differences in the content and relative content of GSs among varieties and organs. In editable roots, the total GSs were 22.59, 12.44 and 27.94 mg·g^-1DM and the relative content of total aliphatic GSs were above 95% in three radish varieties, respectively. The content of total GSs and total aliphatic GSs in edible roots were 2.6-4.9 and 2.8-6.0 times higher than those in leaves of three varieties, respectively. The content of total indolyl GSs in edible roots of ‘Chuzhou xiaowuying' was 1.7 times higher than that in leaves, while in ‘Guling xialuobo' and ‘Yunnan bailuobo'the content of total indolyl GSs in editable roots were only 0.37 times and 0.77 times as much as those in leaves. The relative contents of the major GS of 4-methylthio-3-butenyl GS were accounted for 90.43%-93.92% in edible roots and 62.42%-74.05% in leaves. These results suggested that the cruciferous vegetable of radish was rich in GSs and there were significant variations of the content of GSs in different genotypes and organs．