朱鹮人工饲养群体扩增DNA指纹分析

利用 2 0个 10碱基的随机引物对陕西朱保护站人工饲养的 10只朱 (N ipponia nippon)进行了DNA扩增。结果表明 ,其中 16个引物对所测个体产生了一致的扩增 DNA带纹 ,4个引物产生的 DNA带纹具有多态性。据此计算了扩增 DNA指纹条带的平均带纹相似率为 0 .85 ,平均杂合度为 0 .5 4;在此基础上 ,分析了 10只朱的亲缘关系关系并绘制了相关关系聚类图

Analysis on the amplified DNA fingerprints in feeding population of crestedibises (Nipponia nippon)

The DNAs of 10 feeding crested ibises ( Nipponia nippon ) in crested ibis protection station of Shaanxi province were amplified with 20 10 mer base primers.The results showed that the amplified products within each of the 16 random primers produced identical band patterns of electrophoresis in 10 feeding crested ibises.Only 4 primers amplified polymporphic DNA fingerprints.Hereby,average band sharing rate and probability for a band to be present in the heterozygous state in 10 crested ibises were calculated.They were 0.85 and 0.54 respectively.Based on the average band sharing rate,the blood relationship of ten crested ibises was analyzed and the clustering map of relationship of the ten crested ibises was obtained according to average band sharing rate.