不同激素配比对重瓣矮牵牛组织培养的影响 |
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引用本文: | 宗宪春,宗灿华,刘静,司徒琳莉,任如意,佟亚辉. 不同激素配比对重瓣矮牵牛组织培养的影响[J]. 安徽农业科学, 2010, 38(17): 8877-8878,9102. DOI: 10.3969/j.issn.0517-6611.2010.17.018 |
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作者姓名: | 宗宪春 宗灿华 刘静 司徒琳莉 任如意 佟亚辉 |
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作者单位: | 牡丹江师范学院生命科学与技术学院,黑龙江牡丹江,157012;牡丹江医学院,黑龙江牡丹江,157011 |
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基金项目: | 黑龙江省教育厅科研项目 |
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摘 要: | [目的]研究不同激素配比对重瓣矮牵牛组织培养的影响。[方法]以重瓣矮牵牛叶片为外植体,研究不同激素配比对其愈伤组织、不定芽诱导和不定芽生根的影响。[结果]愈伤组织及不定芽的诱导以MS+6-BA 2.0 mg/L+NAA 0.2 mg/L最佳,不定芽生根以1/2 MS+NAA 0.1 mg/L或1/2MS+IBA 0.1 mg/L最佳,产生的根较粗,数量较多。[结论]该研究结果为重瓣矮牵牛的快速繁殖、种质资源的保存和遗传转化建立了基础。
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关 键 词: | 重瓣矮牵牛 激素配比 组织培养 |
Effect of the Plant Hormone Ratio on Tissue Culture of Petunia hybrida |
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Affiliation: | ZONG Xian-chun et al(College of Life Science and Technology,Mudanjiang Teachers College,Mudanjiang,Heilongjiang 157012) |
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Abstract: | [Objective]The study aimed to research the effect of plant hormone ratio on tissue culture of Petunia hybrida.[Method] Leaves of Petunia hybrida was used for explant to study effect of plant hormone ratio on callus,bud induction and root formation.[Result] The results showed that MS+6-BA 2.0 mg/L+ NAA 0.2 mg/L was suited for callus and bud induction;1/2 MS+NAA 0.1 mg/L or 1/2 MS+IBA 0.1 mg/L were suited for root formation.[Conclusion]It provided basic data for the establishment of rapid propogation,indigenous resources conservation and genetic transformation of Petunia hybrida. |
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Keywords: | Petunia hybrida Hormone ratio Tissue culture |
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