Enhanced Stem Nematode Resistance of Transgenic Sweetpotato Plants Expressing Oryzacystatin-I Gene |
| |
Authors: | GAO Shang YU Bo ZHAI Hong HE Shao-zhen LIU Qing-chang |
| |
Affiliation: | Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/China Agricultural University, Beijing 100193, P.R.China |
| |
Abstract: | Enhanced stem nematode resistance of transgenic sweetpotato (cv. Lizixiang) was achieved using Oryzacyslatin-I (OCI)gene with Agrobacterium tumefaciens-mediated transformation. A. Tumefaciens strain EHA 105 harbors a binary vector pCAMBIA1301 with OCI gene, gusA gene and hptⅡ gene. Selection culture was conducted using 25 mg L-1 hygromycin. A total of 1715 plants were produced from the inoculated 1 450 cell aggregates of Lizixiang via somatic embryogenesis. GUS assay and PCR analysis of the putative transgenic plants randomly sampled showed that 90.54% of them were transgenic plants. Transgenic plants exhibited significantly enhanced resistance to stem nematodes compared to the untransformed control plants by the field evaluation with stem nematodes. Stable integration of the OCI gene into the genome of resistant transgenic plants was confirmed by Southern blot analysis, and the copy number of integrated OCI gene ranged from 1 to 4. Transgene overexpression in stem nematode-resistant plants was demonstrated by quantitative real-time PCR analysis. This study provides a way for improving stem nematode resistance in sweetpotato. |
| |
Keywords: | Agrobacterium tumefaciens Ipomoea batatas (L.) Lam. Oryzacystatin-I gene stem nematode resistance transgenic plant |
本文献已被 维普 万方数据 ScienceDirect 等数据库收录! |