钙调素对梨自花及异花授粉后花柱自发荧光变化的影响

采用激光共聚焦显微镜研究了外源钙调素及其抗血清对‘丰水’梨自花（不亲和）和异花（亲和）授粉后花柱自发荧光变化的影响。结果表明：授粉12 h后，CaM处理后异花授粉花柱荧光值与未处理异花授粉花柱荧光值相比，花柱上部荧光值降低下部升高；而CaM处理后自花授粉花柱自发荧光值除距柱头2 500～3 000 μm外其余部分均高于未处理自花授粉花柱；CaM抗血清处理后异花授粉花柱荧光值除距柱头1 000～2 000 μm外其余部分均高于未处理异花授粉花柱；CaM抗血清处理自花授粉花柱自发荧光与未处理自花授粉花柱相比，分布规律发生改变，最高值位于距柱头1 000～1 500 μm处。授粉72 h后CaM处理后异花授粉花柱荧光值高于未处理异花授粉花柱，最高值位于子房端；CaM处理后自花授粉与未处理自花授粉花柱自发荧光值相比，分布规律发生改变，最高值位于距柱头1 500～2 000 μm处；CaM抗血清处理后异花及自花授粉花柱自发荧光与未处理异花及自花授粉花柱相比较分布规律均发生了改变，最高值都位于0～500 μm处。证实了外源CaM及其抗血清对自花、异花授粉的生理代谢过程均有影响。

The Variation of Style Auto-fluorescence after Calmodulin Treatment on Self-and Cross-pollination in Pyrus pyrifolia Nakai

Confocal laser scanning microscopy (CLSM)were used to investigate the role of exogenous calmodulin (CaM) and anti-CaM serum in 'Hosui' style auto-fluorescence characterization after self- and cross-pollination for 12 h and 72 h. The results showed that the auto-fluorescence of cross-pollination topside style treated with CaM for 12h reduced and the bottom increased, but the auto-fluorescence of self-pollination style treated with CaM increased except 2 500～3 000 μm behind stigma. Anti-CaM serum treated cross-pollination style increased the whole auto-fluorescence except 2 500～3 000 μm behind stigmaand the tiptop of self-pollination after anti-CaM serum treatment moved to 1 000～1 500 μm behide stigma. After pollination 72 h, the style of cross-pollination treated with CaM increased its auto-fluorescence and the highest value was at ovary. Self-pollination style treated with CaM changed the distributing disciplinarian of auto-fluorescence, its tiptop were at 1 500～2 000 μm behide stigma.The highest auto-fluorescence of self- and cross-pollination treated with anti-CaM serum moved to 0～500 μm behide stigma. This primary evdience showed here confirmed that exogenous calmodulin and anti-CaM serum have effected on metabolize of the self- and cross-pollination.