十字花科植物PIN3基因调控元件及表达分析

为探明十字花科不同物种PIN3基因的表达调控差异,从拟南芥(Arabidopsis thaliala)、红花荠菜(Capsella rubella)、白菜(Brassica rapa)、油菜(Brassica napus)和甘蓝(Brassica oleracea)等17个已完成基因组测序的十字花科物种基因组数据库中鉴定出18个PIN3同源基因。对这18个同源基因编码序列上游1 500bp启动子区域顺式作用元件进行预测及比对分析,结果发现,光响应、多种激素响应及胁迫响应等相关元件呈现出较大差异,其中生长素以及向地性响应相关元件具有较高的保守性。克隆普通荠菜PIN3同源基因(CbPIN3)并构建普通荠菜PIN3基因启动子的GUS报告系统CbPIN3pro::GUS转化拟南芥。与拟南芥基因表达数据库比较,荠菜启动子表达与拟南芥PIN3具有基本一致的组织表达模式,但也存在一定的组织差异,说明植株形态建成中生长素极性转运调控的差异。拟南芥和荠菜PIN3的表达差异是十字花科物种适应性进化模式的缩影。

Regulating elements and expression analysis of PIN3 gene in Brassicaceae family

A total of 18 homologous PIN3 genes were identified in 17 species in Brassicaceae family based on genomic data available including Arabidopsis thaliana,Capsella rubella,Brassica rapa and Brassica oleraceaetc.Gene sequences were subjected to bioinformatics analysis and light-,hormone-response elements and stress-response elements are differently contained in the gene promoter as cis elements.The auxin-response and geotropism response elements are conservative among the 18 homologous genes.The promoter of PIN3 (CbPIN3) from Capsella bursa-pastoris was recombined with GUS reporter system CbPIN3pro::GUS and transformed into Arabidopsis thaliana to investigate the specific expression model of the homologous gene.By comparison of GUS staining,it was found that the CbPIN3 gene displayed same expression pattern with the chip data of PIN3 in Arabidopsis.However,the expression pattern of CbPIN3 varies in some tissue.This might be related to the differentiation of auxin polar transport regulation in plant morphogenesis.The differences in expression pattern of PIN3 between Capsella bursa-pastoris and Arabidopsis thaliana were adaptive evolution in the Brassicaceae family.