Medium optimization for efficient somatic embryogenesis and in vitro plant regeneration of spring common wheat varieties

The efficiency of morphogenesis is investigated in an in vitro culture of common wheat varieties Andros, Lada, Lyuba, Noris, Taezhnaya, and Enita. Data of an analysis of the phytohormonal composition of the medium showed that, for efficient induction of somatic embryogenesis and plant regeneration, it is preferable to use auxin 2,4-D and Dicamba rather than 2,4,5-T and 4-CPA since they don’t have a sufficient stimulating effect on wheat tissues. Variation of the carbohydrate composition of the nutrient medium showed that the addition of various sources (sucrose, maltose, glucose) at a certain concentration can equally efficiently stimulate a morphogenic response in all studied wheat varieties.