| 烤烟叶片多酚氧化酶和抗坏血酸氧化酶活性影响因素研究 |
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| 引用本文： | 韩富根,韩锦峰,赵铭钦,许自成,刘学芝. 烤烟叶片多酚氧化酶和抗坏血酸氧化酶活性影响因素研究[J]. 河南农业大学学报, 2004, 38(4): 432-435 |
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| 作者姓名： | 韩富根 韩锦峰 赵铭钦 许自成 刘学芝 |
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| 作者单位： | 河南农业大学农学院,河南,郑州,450002 |
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| 基金项目： | 国家烟草专卖局资助项目(11019990100) |
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| 摘 要： | 研究了一些理化因素对烤烟叶片多酚氧化酶和抗坏血酸氧化酶活性的影响．结果表明，2种酶均较耐高温，但抗坏血酸氧化酶热稳定性好于多酚氧化酶．多酚氧化酶作用最适pH值为6，抗坏血酸氧化酶为5．Cu^2 能增强多酚氧化酶活性，减弱抗坏血酸氧化酶活性．EDTA，NaHSO3，H3BO3，柠檬酸既是多酚氧化酶的良好抑制剂，又是抗坏血酸氧化酶的激活剂．
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| 关 键 词： | 烤烟 叶片 多酚氧化酶 抗坏血酸氧化酶 酶活性 |
| 文章编号： | 1000-2340(2004)04-0432-04 |
Study on the influencing factors of polyphenol oxidase and ascorbic oxidase activities of flue-cured tobacco leaves |
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| HAN Fu-gen,HAN Jin-feng,ZHAO Ming-qin,XU Zi-cheng,LIU Xue-zhi. Study on the influencing factors of polyphenol oxidase and ascorbic oxidase activities of flue-cured tobacco leaves[J]. Journal of Henan Agricultural University, 2004, 38(4): 432-435 |
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| Authors: | HAN Fu-gen HAN Jin-feng ZHAO Ming-qin XU Zi-cheng LIU Xue-zhi |
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| Abstract: | This paper studied the influence of a few physical and chemical factors on the activities of polyphenol oxidase and ascorbic acid oxidase in flue-cured tobacco leaves. Results showed that the two oxidases both could resist high temperature, but the heat stability of ascorbic acid oxidase is higher than that of polyphenol oxidase. Polyphenol oxidase and ascorbic acid oxidase had their optimum at pH6 and pH5, respectively. Cu~(2+) could enhance the activity of polyphenol oxidase but weaken the activity of ascorbic acid oxidase. EDTA, NaHSO_3, H_3BO_3 and citric acid were the effective inhibitors of polypenol oxidase, but were the activators of ascorbic acid oxidase. |
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| Keywords: | flue-cured tobacco leaves polypenol oxidase ascorbic acid oxidase enzyme properties |
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