小麦雌蕊和雄蕊突变体与川麦28之间特异性ISSR标记筛选

利用42条ISSR标记对小麦三雌蕊突变体(TP)和雄蕊同源转化为雌蕊突变体(HTS-1)与川麦28之间特异性的ISSR分子标记进行筛选,为利用ISSR标记定位Pis1基因和控制雄蕊同源转化成雌蕊的hts1和hts2基因奠定基础.结果表明:42条引物共扩增403个条带,有9条引物能在TP和CM 28之间扩增出差异条带,占所用引物的21.4％.有20条引物能在HTS-1和CM 28之间扩增出差异条带,占所用引物的47.6％.有21条引物能在TP与HTS-1之间扩增出差异条带,占所用引物的50％.每条引物最多能扩增出4条差异条带,大部分产生1～2条差异性条带.差异条带大小主要集中在250～750 bp之间.这也证明了ISSR标记是一种简单有效的分子标记,可作为SSR的一种重要补充标记用于遗传图谱的构建.

Screening of Specific Markers Among Wheat Line Three Pistils,Pistillody and Chuanmai 28 by ISSR Analysis

In order to develop specific molecular markers between common wheat three pistils mutant (TP),pistillody mutant (HTS-1) and Chuanmai 28(CM 28),and lay the foundation for fine mapping of Pis1,hts1 and hts2 gene,the genetic diversities of TP,HTS-1 and CM 28 were compared and analyzed by using inter-simple sequence repeat (ISSR) marker.The results indicated that all the 42 ISSR primers amplified 403 bands.Nine ISSR primers (21.4％) were polymorphic between TP and CM 28.Twenty ISSR primers (47.6 ％) were polymorphic between HTS-1 and CM 28,and 21 primers (50 ％) were polymorphic between TP and HTS-1.A maximum of 4 different bands can be amplified by each primer and most of which produced 1-2 different bands.The difference bands were mainly with size in the range of 250-750 bp.These results showed that ISSR was simple,economic and effective molecular marker,and can be used as an important complement of SSR markers for the genetic map construction in wheat.