黑木相思根蘖促萌及组培繁育技术研究

文章以根蘖促萌和组织培养相结合的方法研究黑木相思优良无性系快繁技术，4个参试的无性系都获得了根蘖萌条，最高可达12条／根。采集10～20cm的萌条作外植体进行组培快繁时，灭菌成功率较高，最高可达80．9％。试验结果表明以根蘖萌条为外植体诱导的组培苗生根性能好，在黑木相思组培增殖中有效防止苗木发黄和促进不定芽叶片舒展是两个关键因子，通过降低培养基的含糖量是一种有效的途径，由此经3次继代培养无菌苗数量平均达23株／瓶。

Study on Propagation Technology about Root Sprout and Tissue Culture of Acacia melanoxylon

A method combined the root sprout and tissue culture were applied in the rapid propagation of clones of Acacia melanoxylon. Four tested clones were all cultured the root sprout, up to 12 sprouts. High efficien- cy of sterilization, up to 80.9 percent, was found when 10 -20 cm root sprout were propagated as explant. The re- suits showed that good performance with rhizogenesis as explants were root sprouts. Two key factors, the prevention of yellow leaves and the promotion of adventitious buds stretch, must be noticed in the proliferation. Decreasing the sugar content of substrate was an effective approach. In this way, the number of asepsis sprouts were average 23 each bottle with the three step-gap culture.