| 黄瓜抗枯萎病离体鉴定技术的研究 |
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| 引用本文： | 赵秀娟,吴定华.黄瓜抗枯萎病离体鉴定技术的研究[J].华南农业大学学报,2001,22(1):39-41,45. |
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| 作者姓名： | 赵秀娟 吴定华 |
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| 作者单位： | 华南农业大学园艺系, |
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| 摘 要： | 通过比较10种具有不同枯萎病抗怀的黄瓜材料对两类选择压力的反应，确定离体抗鉴定的适应选择压力为FOC（the culture filtraltes of Fusarium oxysporum f.sp.cucumerinum)原液和1mg/L FA(fusaric acid)粗制品溶液，适宜的鉴定时间为愈伤组织诱导期，黄瓜材料离体培养物对选择压力的反应与材料苗期抗病性比较一致。
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| 关 键 词： | 黄瓜 枯萎病 离体鉴定 抗性鉴定 苗期鉴定 组织培养 选择压力 愈伤组织诱导期 抗病育种 |
| 文章编号： | 1001-411X (2001) 01-0039-03 |
Studies on Techniques of in vitro Evaluation of Fusaric Wilt-Resistance in Cucumber |
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| ZHAO Xiujuan,WU Dinghua.Studies on Techniques of in vitro Evaluation of Fusaric Wilt-Resistance in Cucumber[J].Journal of South China Agricultural University,2001,22(1):39-41,45. |
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| Authors: | ZHAO Xiujuan WU Dinghua |
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| Abstract: | Influence of two kinds of selection agents on the tissue culture of ten genotypes with different resistance to fusarium wilt in cucumber were studied .The results showed that the suitable agents of in vitro evaluation were the culture filtrates of Fusarium oxysporum f.sp. cucumerinum(FOC)and 1 mg/L crude fusaric acid (FA )extracts ,and the suitable period for identification was the induction of callus phase .The tissue culture resistance to agents showed consistence with the seedling resistance to the pathogen . |
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| Keywords: | cucumber fusaric wilt resistance evaluation in vitro tissue culture selection agent |
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