| 应用昆虫杆状病毒载体在昆虫细胞中表达乙肝表面抗原 |
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| 引用本文： | 周耐明,宓怡德,金伟,李载平,吴祥甫. 应用昆虫杆状病毒载体在昆虫细胞中表达乙肝表面抗原[J]. 浙江大学学报(农业与生命科学版), 1995, 0(1) |
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| 作者姓名： | 周耐明 宓怡德 金伟 李载平 吴祥甫 |
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| 作者单位： | 浙江农业大学蚕学系，中国科学院上海生物化学研究所 |
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| 摘 要： | 人乙型肝炎病毒（ａｄｒ亚型）表面抗原Ｓ基因克隆到ＰｕＡＣ－５苜蓿尺蠖核型多角体病毒转移载体上，所构建的重组转移载体质粒与野生型ＡｃＮＰＶＤＮＡ共转染Ｓｆ－２１细胞，经空斑法筛选和纯化，得到了含ＨＢＶＳ基因的重组病毒ＡｃＮＰＶＳ．用放射免疫法测定了ＡｃＮＰＶＳ感染的Ｓｆ－２１细胞及其培养液中的ＨＢｓＡｇ，总表达量为１．２２μｇ／１０￣６细胞．
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| 关 键 词： | 苜蓿尺蠖核型多角体病毒；乙型肝炎病毒表面抗原；昆虫细胞；基因表达 |
Expression of hepatitis B virus surface antigen in insect cells using a baculovirus vector |
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| Zhou Naiming,Mz Yide, Jin Wei,Li zaiping,Wu Xiangfu. Expression of hepatitis B virus surface antigen in insect cells using a baculovirus vector[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 1995, 0(1) |
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| Authors: | Zhou Naiming Mz Yide Jin Wei Li zaiping Wu Xiangfu |
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| Abstract: | The S gene sequence from HBV(adr sub-type)was inserted into a Autographa cali-fornica nuclear polyhedrosis virus(AcNPV ) vector PuAC 5 to construct a recombinant transfervector plasmid PuACHS-5. The Sf-21 cells were co-transfected with PuACHS-5 DNA and wild-type Ac NPV DNA.Arecombinant virus AcNPVS was selected and purified by plaque assay. Thelevel of HBsAg expression in Sf-21 ceIls infected with recombinant virus AcNPVS was estimated atatotal of l.22 t1gl106 cells by radioimmunoassay. |
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| Keywords: | Autographa californica nuclear polyhedros is virus hepatitis B virus surface anti-gen insect cell gene expression |
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