| 一例鸭疫里默氏杆菌病的实验室诊断 |
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| 引用本文： | 刘丽娟,马光强,吴良涛,杨均,张海莉,刘军泽,陈国权,王开功,周碧君.一例鸭疫里默氏杆菌病的实验室诊断[J].贵州畜牧兽医,2020,44(3):49-51. |
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| 作者姓名： | 刘丽娟 马光强 吴良涛 杨均 张海莉 刘军泽 陈国权 王开功 周碧君 |
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| 作者单位： | 贵州省都匀市农业农村局,贵州 都匀 558000,黔南民族职业技术学院,贵州 都匀 558000,黔南民族职业技术学院,贵州 都匀 558000,黔南民族职业技术学院,贵州 都匀 558000,黔南民族职业技术学院,贵州 都匀 558000,黔南民族职业技术学院,贵州 都匀 558000,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025 |
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| 摘 要： | 为确诊贵州省三穗县某养鸭场疑似鸭疫里默氏杆菌感染病例,通过实验室细菌分离培养、生化试验、PCR检测及基因测序对病原进行鉴定,并进行药物敏感性试验。结果:分离细菌为革兰氏阴性杆菌;生化特征与鸭疫里默氏杆菌相符;PCR检测及基因测序与鸭疫里默氏杆菌OmpA基因同源性达100%;药敏试验对卡那霉素、新霉素、哌拉西林3种抗菌素高度敏感。结论:确诊病例为鸭疫里默氏杆菌病,可根据药敏试验结果选择高敏药物进行防治。
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| 关 键 词： | 鸭疫里默氏杆菌 细菌分离鉴定 PCR检测 药敏试验 |
A Case of Laboratory Diagnosis of Infectious Serositis of Duck |
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| Liu Lijuan,Ma Guangqiang,Wu Liangtao,Yang Jun,Zhang Haili,Liu Junze,Chen Guoquan,Wang Kaigong,Zhou Bijun.A Case of Laboratory Diagnosis of Infectious Serositis of Duck[J].Guizhou Animal Science and Veterinary Medicine,2020,44(3):49-51. |
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| Authors: | Liu Lijuan Ma Guangqiang Wu Liangtao Yang Jun Zhang Haili Liu Junze Chen Guoquan Wang Kaigong Zhou Bijun |
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| Institution: | (Agriculture and Rural Bureau of Duyun City,Guizhou Province,Duyun Guizhou 558000,China;Qiannan Vocational College of Nationalities,Duyun Guizhou 558000,China;College of Animal Science,Guizhou University,Guiyang Guizhou 550025,China) |
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| Abstract: | In order to confirm the suspected case of Riemerella anatipestifer infection in a duck farm in Sansui county,Guizhou province,the pathogens were identified through laboratory bacterial isolation and culture,biochemical tests,PCR testing and gene sequencing,and drug sensitivity tests were conducted.Results:The isolated bacteria were Gram-negative bacilli;the biochemical characteristics were consistent with Riemerella anatipestifer;PCR detection and gene sequencing were 100%homologous to the OmpA gene of Riemerella anatipestifer;3 antibiotics,neomycin,piperacillin are highly sensitive.Conclusion:the confirmed case is Riemerella anatipestifer,and high-sensitivity drugs can be selected for prevention and treatment according to the results of drug susceptibility tests. |
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| Keywords: | Riemerella anatipestifer Isolation and Identification PCR Detection Drug Sensitivity Test |
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