| 一例鸡马立克氏病的病原学诊断 |
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| 引用本文： | 尹德晶,陈静,杨鹏,谢晓东,李乔斌,王开功,程振涛,岳筠.一例鸡马立克氏病的病原学诊断[J].贵州畜牧兽医,2020,44(2):35-37. |
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| 作者姓名： | 尹德晶 陈静 杨鹏 谢晓东 李乔斌 王开功 程振涛 岳筠 |
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| 作者单位： | 贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025;贵州省动物疫病研究室,贵州 贵阳 550025,贵州大学动物科学学院,贵州 贵阳 550025;贵州省动物疫病研究室,贵州 贵阳 550025,贵州省动物疫病预防控制中心,贵州 贵阳 550008 |
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| 基金项目： | "贵州省预防兽医专业硕士研究生工作站"贵州省研究生教育创新计划项目;"贵州省动物疫病防控与兽医公共卫生保障科技创新人才团队"贵州省科技创新人才团队项目 |
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| 摘 要： | 为确诊贵州省福泉市某养殖场鸡群死亡病因,采集病鸡的组织病料进行细菌分离培养,同时采用普通PCR和荧光定量PCR方法对组织病料进行相关病原核酸检测。结果:病鸡组织中未分离出细菌;病原核酸检测显示,马立克氏病病毒阳性,新城疫病毒、禽流感病毒、鸡滑液囊支原体均呈阴性。结论:确诊病例为马立克氏病病毒感染。
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| 关 键 词： | 鸡 马立克氏病 病原检测 诊断 |
Etiological Diagnosis of A Case of Marek's Disease in Chickens |
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| Ying Dejing,Chen Jing,Yang Peng,Xie Xiaodong,Li Qiaobin,Wang Kaigong,Cheng Zhentao,Yue Jun.Etiological Diagnosis of A Case of Marek's Disease in Chickens[J].Guizhou Animal Science and Veterinary Medicine,2020,44(2):35-37. |
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| Authors: | Ying Dejing Chen Jing Yang Peng Xie Xiaodong Li Qiaobin Wang Kaigong Cheng Zhentao Yue Jun |
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| Institution: | (College of Animal Science,Guizhou University,Guiyang Guizhou 550025,China;Animal Epidemic Disease Research Office of Guizhou Province,Guiyang Guizhou 550025,China;Animal Disease Prevention and Control Center in Guizhou Province,Guiyang Guizhou 550008,China) |
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| Abstract: | In order to confirm the cause of death of chickens in a farm in Fuquan city,Guizhou province,the tissue disease materials of sick and dead chickens were collected for bacterial isolation and culture,and the related pathogenic nucleic acids were detected by ordinary PCR and fluorescence quantitative PCR.Results:no bacteria were isolated from diseased chicken tissues,and the detection of pathogenic nucleic acid showed that Marek's disease virus was positive,while Newcastle disease virus,avian influenza virus and chicken synovial sac mycoplasma were negative.Conclusion:the confirmed case is Marek's disease virus infection. |
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| Keywords: | Chicken Marek's Disease Pathogen Detection Diagnosis |
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