高压静电场对乐东拟单性木兰插穗生根的影响及其生理机制

以乐东拟单性木兰为试材,研究了2.5、5、7.5和10 k V·cm-1不同场强的静电场对插穗生根及相关生理指标的影响。结果表明,5 k V·cm-1的静电处理其插穗的平均单株生根数、根均长、根鲜重、根系活力和成活率均高于其他处理,与对照相比分别提高了83.3%、59.55%、102.9%、96.16%和131.34%;对可溶性糖、可溶性蛋白、过氧化物酶(POD)和吲哚乙酸氧化酶(IAAO)的检测发现,除IAAO活性变化呈现先下降后缓慢升高的趋势外,其他指标的变化均总体呈现先升后降的趋势,在15 d时达到峰值,其中,5 k V·cm-1处理其可溶性糖含量、可溶性蛋白含量、POD活性分别较对照升高了38.55%、45.96%和57%;而IAAO酶与对照相比降低了62.47%。结果表明,静电场处理能显著提高乐东拟单性木兰插穗的扦插成活率,最高值达到了50.71%,其最佳静电场场强为5 k V·cm-1。

Effects of the high voltage electrostatic field on rooting of Parakmeria lotungensis cuttings and related physiology mechanisms

The effects of different intensities of the electrostatic field with 2.5, 5, 7.5 and 10 kV·cm^-1 on rooting of Parakmeria lotungensis cuttings were investigated and the corresponding physiology mechanisms were also studied. The results showed that the average root number, root length, root fresh weight, root activity and survival rate of cuttings with 5 kV·cm^-1 electrostatic field treatment were higher than those in other treatments with an increase of 83.3%, 59.55%, 102.9%, 59.55% and 131.34%, respectively compared to CK. The content of soluble protein, soluble sugar and the activities of POD and IAAO of the cuttings were also detected. Results showed that the IAAO activity slowly increased first, then decreased, while the other corresponding indexes were first decreased and then increased and and the index reached the maximum at 15 days after treatment. The soluble sugar, soluble protein and POD activity with 5 kV·cm^-1 electrostatic field treatment increased by 38.55%, 45.96% and 57%, respectively, and IAAO enzyme activity decreased by 62.47% compared with CK. The results showed that the electrostatic field treatment can significantly improve the survival rate of Parakmeria lotungensis cuttings, and the best intensity of electrostatic field was 5 kV·cm^-1 and the survival rate of cuttings could reach to 50.71%.