生长素对小麦幼胚组织培养的影响

以川农16、川麦42和良麦2号为供试材料,探讨了三种生长素对其幼胚组织培养的影响,并在此基础上筛选出相对较好的受体材料。结果表明,三种生长素在幼胚的诱导率、胚发芽率和胚发根率上存在极显著差异。2,4,5-T和NAA在诱导小麦幼胚培养基,其胚芽、胚根发生严重;而2,4-D在诱导率、胚发芽率和胚发根率这三方面相对优于2,4,5-T和NAA,适用于小麦幼胚的组织培养,且适宜浓度范围在2.0~2.5 mg/L。基因型对小麦幼胚组织培养的诱导率没有影响,对胚发芽率有一定影响,而对胚发根率有较大影响。在三种基因型中,以川农16在诱导率、胚发芽率和胚发根率这三方面表现相对突出,可作为转化受体材料。

Effect of auxins on the tissue culture from immature embryos of wheat

To screen out the desirable genotypes and auxins for tissue culture,the influences of 3 auxins on the tissue culture from the immature embryos of 3 genotypes(ie.Chuannong 16,Chuanmai 42 and Liangmai 2) were investigated.It was found that 3 auxins had the significant effects on the frequency of immature embryos callus induction,germination and rootage.The addition of 2,4,5-T and NAA in the media resulted in a great deal of immature embryos germination and rootage.It was observed that 2,4-D was superior to 2,4,5-T and NAA in the frequency of immature embryos callus induction,germination and rootage.Optimum content of 2,4-D for the tissue culture for the immature embryos of wheat was 2.0-2.5 mg/L.Genotype had no effect on frequency of immature embyos callus induction,while it had the significant effect on frequency of germination,and frequency of rootage.Among the 3 genotypes,Chuannong 16 had the best performance in the frequency of immature embryos callus induction,germination and rootage,thus it could be used in the wheat genetic transformation.