| ITS序列及其SNP位点在外来入侵杂草长芒苋、西部苋和糙果苋物种鉴定中的应用 |
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| 引用本文： | 徐 晗,赵彩云,刘勇波,陈鹏程,李俊生. ITS序列及其SNP位点在外来入侵杂草长芒苋、西部苋和糙果苋物种鉴定中的应用[J]. 植物保护, 2017, 43(5): 128-133. DOI: 10.3969/j.issn.0529-1542.2017.05.021 |
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| 作者姓名： | 徐 晗 赵彩云 刘勇波 陈鹏程 李俊生 |
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| 作者单位： | 1. 中国环境科学研究院,北京100012;中国检验检疫科学研究院,北京100176;2. 中国环境科学研究院,北京,100012;3. 绍兴出入境检验检疫局,绍兴,312000 |
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| 基金项目： | 国家重点研发计划(2016YFC1201105); 中国检验检疫科学研究院基本科研业务费项目(2014JK010, 2017JK038) |
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| 摘 要： | 对于以种子形态为物种主要识别依据的口岸部门,外来入侵杂草长芒苋、西部苋和糙果苋一直是鉴定的难点。本文对34种苋属植物的ITS序列和26SrDNA进行分析,通过SNP变异位点及特异性引物,借助PCR-RFLP方法,对长芒苋、西部苋与糙果苋进行快速、准确的分类鉴定。ITS序列分析表明,长芒苋种内变异小,种间变异显著,可与其他苋属植物明显区分。西部苋与糙果苋之间ITS序列差异小,需依据SNP位点来区别。
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| 关 键 词： | ITS序列 异株苋亚属 长芒苋 西部苋 糙果苋 26S rDNA SNP PCR-RFLP |
| 收稿时间： | 2016-12-13 |
| 修稿时间： | 2017-01-30 |
Taxonomic circumscription of Amaranthus palmeri, A.rudis and A.tuberculatus (Amaranthaceae) based on ITS sequences and SNP analysis |
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| Xu Han,Zhao Caiyun,Liu Yongbo,Chen Pengcheng,Li Junsheng. Taxonomic circumscription of Amaranthus palmeri, A.rudis and A.tuberculatus (Amaranthaceae) based on ITS sequences and SNP analysis[J]. Plant Protection, 2017, 43(5): 128-133. DOI: 10.3969/j.issn.0529-1542.2017.05.021 |
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| Authors: | Xu Han Zhao Caiyun Liu Yongbo Chen Pengcheng Li Junsheng |
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| Affiliation: | 1. Chinese Academy of Environmental Sciences, Beijing 100012, China; 2. Chinese Academy of Inspection and Quarantine, Beijing 100176, China; 3. Shaoxing Entry-Exit Inspection and Quarantine Bureau, Zhejiang 312000, China |
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| Abstract: | Alien invasive weeds Amaranthus palmeri, A.rudis and A.tuberculatus are difficult to distinguish from each other, and taxonomical identification only depends on seed morphologies. In this study, A.palmeri, A.rudis and A.tuberculatus could be identified quickly and precisely through PCR-RFLP method, based on ITS and 26S rDNA analyses, and specific primers designed by SNP. The results show that there is no intraspecific variation within A.palmeri populations, but interspecific variation is significant. A.palmeri can be differentiated from other congeneric species by ITS.However, differences of ITS sequences between A.rudis and A.tuberculatus are small, they should be defined further by SNP analysis. |
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| Keywords: | ITS subgen. Acnida Amaranthus palmeri Amaranthus rudis Amaranthus tuberculatus 26S rDNA SNP PCR-RFLP |
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